WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience Synaptic Systems Antibody Company
 QUICK SEARCH:   [advanced]


     
-


HOME
  |  
SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, November 8, 2006, 26(45):11753-11762; doi:10.1523/JNEUROSCI.2275-06.2006

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via ISI Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Priller, J.
Right arrow Articles by Aguzzi, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Priller, J.
Right arrow Articles by Aguzzi, A.

 Previous Article  |  Next Article 

Neurobiology of Disease
Early and Rapid Engraftment of Bone Marrow-Derived Microglia in Scrapie

Josef Priller,1,2 * Marco Prinz,1,3 * Mathias Heikenwalder,1 * Nicolas Zeller,1 Petra Schwarz,1 Frank L. Heppner,1 and Adriano Aguzzi1

1Institute of Neuropathology, Department of Pathology, University of Zurich, 8091 Zurich, Switzerland, 2Departments of Psychiatry and Experimental Neurology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany, and 3Institute of Neuropathology, University of Göttingen, 37075 Göttingen, Germany

Correspondence should be addressed to either of the following: Adriano Aguzzi, Institute of Neuropathology, University of Zurich, Schmelzbergstrasse 12, CH-8091 Zurich, Switzerland Email: adriano.aguzzi{at}usz.ch; or Josef Priller, Departments of Psychiatry and Experimental Neurology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany Email: josef.priller{at}charite.de

Prion neuroinvasion is accompanied by maximal activation of microglia, the significance of which for pathogenesis is unknown. Here, we used bone marrow (BM) cells expressing GFP (green fluorescent protein) to study the turnover of microglia in mouse scrapie. We found that ≥50% of all brain microglia were replaced by BM-derived cells before clinical disease onset. In terminally sick mice, microglia density increased threefold to fourfold. Hence BM-derived microglia rapidly and efficaciously colonize the brain in scrapie. Whereas reconstitution of wild-type mice with prion protein-deficient (Prnpo/o) BM did not alter scrapie pathogenesis, Prnpo/o mice transplanted with wild-type BM cells were resistant to peripherally administered prions despite high levels of infectivity in the spleen. Cerebellar homogenates from prion-inoculated Prnpo/o mice reconstituted with >10% of wild-type microglia failed to infect transgenic mice overexpressing the cellular prion protein. Hence, in contrast to previous reports, microglia are not competent for efficient prion transport and replication in vivo.

Key words: prion infectivity; microglia; bone marrow transplantation; green fluorescent protein; Purkinje cells; knock-out


Received May 29, 2006; revised Sept. 26, 2006; accepted Sept. 27, 2006.

Correspondence should be addressed to either of the following: Adriano Aguzzi, Institute of Neuropathology, University of Zurich, Schmelzbergstrasse 12, CH-8091 Zurich, Switzerland Email: adriano.aguzzi{at}usz.ch; or Josef Priller, Departments of Psychiatry and Experimental Neurology, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany Email: josef.priller{at}charite.de






-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help

-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-