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The Journal of Neuroscience, April 11, 2007, 27(15):4027-4035; doi:10.1523/JNEUROSCI.0462-07.2007

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Cellular/Molecular
Glutamatergic and Purinergic Receptor-Mediated Calcium Transients in Bergmann Glial Cells

Richard Piet and Craig E. Jahr

Vollum Institute, Oregon Health & Science University, Portland, Oregon 97239

Correspondence should be addressed to Craig E. Jahr, Vollum Institute L-474, Oregon Health & Science University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97239-3098. Email: jahr{at}ohsu.edu

Astrocytes respond to neuronal activity with [Ca²⁺]_i increases after activation of specific receptors. Bergmann glial cells (BGs), astrocytes of the cerebellar molecular layer (ML), express various receptors that can mobilize internal Ca²⁺. BGs also express Ca²⁺ permeable AMPA receptors that may be important for maintaining the extensive coverage of Purkinje cell (PC) excitatory synapses by BG processes. Here, we examined Ca²⁺ signals in single BGs evoked by synaptic activity in cerebellar slices. Short bursts of high-frequency stimulation of the ML elicited Ca²⁺ transients composed of a small-amplitude fast rising phase, followed by a larger and slower rising phase. The first phase resulted from Ca²⁺ influx through AMPA receptors, whereas the second phase required release of Ca²⁺ from internal stores initiated by P2 purinergic receptor activation. We found that such Ca²⁺ responses could be evoked by direct activation of neurons releasing ATP onto BGs or after activation of metabotropic glutamate receptor 1 on these neurons. Moreover, examination of BG and PC responses to various synaptic stimulation protocols suggested that ML interneurons are likely the cellular source of ATP.

Key words: cerebellum; neuron–glia interaction; ATP; AMPA receptors; mGluR1; synaptic transmission; interneuron

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Received Aug. 8, 2006; accepted March 10, 2007.

Correspondence should be addressed to Craig E. Jahr, Vollum Institute L-474, Oregon Health & Science University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97239-3098. Email: jahr{at}ohsu.edu

Related articles in J. Neurosci.:

Molecular Layer Interneurons Relay Cerebellar Cortical Activity to Bergmann Glial Cells

Tycho M. Hoogland, Eugene F. Civillico, and Bernd Kuhn
J. Neurosci. 2007 27: 11167-11169. [Full Text]  

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				K. Caesar, P. Hashemi, A. Douhou, G. Bonvento, M. G. Boutelle, A. B. Walls, and M. Lauritzen Glutamate receptor-dependent increments in lactate, glucose and oxygen metabolism evoked in rat cerebellum in vivo J. Physiol., March 1, 2008; 586(5): 1337 - 1349. [Abstract] [Full Text] [PDF]

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