QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, May 9, 2007, 27(19):5224-5235; doi:10.1523/JNEUROSCI.5169-06.2007

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Related articles in J. Neurosci. Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (41) Citing Articles via Google Scholar Google Scholar Articles by Cancedda, L. Articles by Poo, M.-m. Search for Related Content PubMed PubMed Citation Articles by Cancedda, L. Articles by Poo, M.-m.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Excitatory GABA Action Is Essential for Morphological Maturation of Cortical Neurons In Vivo

Laura Cancedda, ^* Hubert Fiumelli, ^* Karen Chen, and Mu-ming Poo

Division of Neurobiology, Department of Molecular and Cell Biology, Helen Wills Neuroscience Institute, University of California at Berkeley, Berkeley, California 94720-3200

Correspondence should be addressed to Mu-ming Poo at the above address. Email: mpoo{at}berkeley.edu

GABA exerts excitatory actions on embryonic and neonatal cortical neurons, but the in vivo function of this GABA excitation is essentially unknown. Using in utero electroporation, we eliminated the excitatory action of GABA in a subpopulation of rat ventricular progenitors and cortical neurons derived from these progenitors by premature expression of the Cl^– transporter KCC2, as confirmed by the changes in the reversal potential of GABA-induced currents and the resting membrane potential after GABA_A receptor blockade. We found that radial migration to layer II/III of the somatosensory cortex of neurons derived from the transfected progenitors was not significantly affected, but their morphological maturation was markedly impaired. Furthermore, reducing neuronal excitability of cortical neurons in vivo by overexpressing an inward-rectifying K⁺ channel, which lowered the resting membrane potential, mimicked the effect of premature KCC2 expression. Thus, membrane depolarization caused by early GABA excitation is critical for morphological maturation of neonatal cortical neurons in vivo.

Key words: KCC2; development; radial migration; K_ir2.1; in utero electroporation; gramicidin perforated-patch

---

Received Nov. 29, 2006; revised March 7, 2007; accepted March 26, 2007.

Correspondence should be addressed to Mu-ming Poo at the above address. Email: mpoo{at}berkeley.edu

Related articles in J. Neurosci.:

The Influence of GABA on Dendritic Development In Vivo

Winnie Wefelmeyer
J. Neurosci. 2007 27: 10649-10650. [Full Text]  

This article has been cited by other articles:

				N. Ageta-Ishihara, S. Takemoto-Kimura, M. Nonaka, A. Adachi-Morishima, K. Suzuki, S. Kamijo, H. Fujii, T. Mano, F. Blaeser, T. A. Chatila, et al. Control of Cortical Axon Elongation by a GABA-Driven Ca2+/Calmodulin-Dependent Protein Kinase Cascade J. Neurosci., October 28, 2009; 29(43): 13720 - 13729. [Abstract] [Full Text] [PDF]

				R. Wright The Necessity of NKCC1: Loss of the Chloride Cotransporter in a Knock-Out Model and Potential Compensatory Mechanisms J. Neurosci., October 21, 2009; 29(42): 13094 - 13096. [Full Text] [PDF]

				H. Lu, B. Lim, and M.-m. Poo Cocaine Exposure In Utero Alters Synaptic Plasticity in the Medial Prefrontal Cortex of Postnatal Rats J. Neurosci., October 7, 2009; 29(40): 12664 - 12674. [Abstract] [Full Text] [PDF]

				H. Fiorentino, N. Kuczewski, D. Diabira, N. Ferrand, M. N. Pangalos, C. Porcher, and J.-L. Gaiarsa GABAB Receptor Activation Triggers BDNF Release and Promotes the Maturation of GABAergic Synapses J. Neurosci., September 16, 2009; 29(37): 11650 - 11661. [Abstract] [Full Text] [PDF]

				J. E. Tanis, A. Bellemer, J. J. Moresco, B. Forbush, and M. R. Koelle The Potassium Chloride Cotransporter KCC-2 Coordinates Development of Inhibitory Neurotransmission and Synapse Structure in Caenorhabditis elegans J. Neurosci., August 12, 2009; 29(32): 9943 - 9954. [Abstract] [Full Text] [PDF]

				R. Jagasia, K. Steib, E. Englberger, S. Herold, T. Faus-Kessler, M. Saxe, F. H. Gage, H. Song, and D. C. Lie GABA-cAMP Response Element-Binding Protein Signaling Regulates Maturation and Survival of Newly Generated Neurons in the Adult Hippocampus J. Neurosci., June 24, 2009; 29(25): 7966 - 7977. [Abstract] [Full Text] [PDF]

				K. T. Kahle, S. M. Barnett, K. C. Sassower, and K. J. Staley Decreased Seizure Activity in a Human Neonate Treated With Bumetanide, an Inhibitor of the Na+-K+-2Cl- Cotransporter NKCC1 J Child Neurol, May 1, 2009; 24(5): 572 - 576. [Abstract] [PDF]

				D. D. Wang and A. R. Kriegstein Defining the role of GABA in cortical development J. Physiol., May 1, 2009; 587(9): 1873 - 1879. [Abstract] [Full Text] [PDF]

				W. Shen, J. S. Da Silva, H. He, and H. T. Cline Type A GABA-Receptor-Dependent Synaptic Transmission Sculpts Dendritic Arbor Structure in Xenopus Tadpoles In Vivo J. Neurosci., April 15, 2009; 29(15): 5032 - 5043. [Abstract] [Full Text] [PDF]

				C. K. Pfeffer, V. Stein, D. J. Keating, H. Maier, I. Rinke, Y. Rudhard, M. Hentschke, G. M. Rune, T. J. Jentsch, and C. A. Hubner NKCC1-Dependent GABAergic Excitation Drives Synaptic Network Maturation during Early Hippocampal Development J. Neurosci., March 18, 2009; 29(11): 3419 - 3430. [Abstract] [Full Text] [PDF]

				C. W. Habela, N. J. Ernest, A. F. Swindall, and H. Sontheimer Chloride Accumulation Drives Volume Dynamics Underlying Cell Proliferation and Migration J Neurophysiol, February 1, 2009; 101(2): 750 - 757. [Abstract] [Full Text] [PDF]

				R. Batista-Brito, R. Machold, C. Klein, and G. Fishell Gene Expression in Cortical Interneuron Precursors is Prescient of their Mature Function Cereb Cortex, October 1, 2008; 18(10): 2306 - 2317. [Abstract] [Full Text] [PDF]

				S. Ge, K. A. Sailor, G.-l. Ming, and H. Song Synaptic integration and plasticity of new neurons in the adult hippocampus J. Physiol., August 15, 2008; 586(16): 3759 - 3765. [Abstract] [Full Text] [PDF]

				E. Y. Rula, A. H. Lagrange, M. M. Jacobs, N. Hu, R. L. Macdonald, and R. B. Emeson Developmental Modulation of GABAA Receptor Function by RNA Editing J. Neurosci., June 11, 2008; 28(24): 6196 - 6201. [Abstract] [Full Text] [PDF]

				D. D. Wang and A. R. Kriegstein GABA Regulates Excitatory Synapse Formation in the Neocortex via NMDA Receptor Activation J. Neurosci., May 21, 2008; 28(21): 5547 - 5558. [Abstract] [Full Text] [PDF]

				V. C. Cuzon, P. W. L. Yeh, Y. Yanagawa, K. Obata, and H. H. Yeh Ethanol Consumption during Early Pregnancy Alters the Disposition of Tangentially Migrating GABAergic Interneurons in the Fetal Cortex J. Neurosci., February 20, 2008; 28(8): 1854 - 1864. [Abstract] [Full Text] [PDF]

				A. Reynolds, E. Brustein, M. Liao, A. Mercado, E. Babilonia, D. B. Mount, and P. Drapeau Neurogenic Role of the Depolarizing Chloride Gradient Revealed by Global Overexpression of KCC2 from the Onset of Development J. Neurosci., February 13, 2008; 28(7): 1588 - 1597. [Abstract] [Full Text] [PDF]

				G. Li, H. Adesnik, J. Li, J. Long, R. A. Nicoll, J. L. R. Rubenstein, and S. J. Pleasure Regional Distribution of Cortical Interneurons and Development of Inhibitory Tone Are Regulated by Cxcl12/Cxcr4 Signaling J. Neurosci., January 30, 2008; 28(5): 1085 - 1098. [Abstract] [Full Text] [PDF]

				B. I. Hutchins and K. Kalil Differential Outgrowth of Axons and their Branches Is Regulated by Localized Calcium Transients J. Neurosci., January 2, 2008; 28(1): 143 - 153. [Abstract] [Full Text] [PDF]

				P. Baldelli, A. Fassio, F. Valtorta, and F. Benfenati Lack of Synapsin I Reduces the Readily Releasable Pool of Synaptic Vesicles at Central Inhibitory Synapses J. Neurosci., December 5, 2007; 27(49): 13520 - 13531. [Abstract] [Full Text] [PDF]

				C.-L. Wang, L. Zhang, Y. Zhou, J. Zhou, X.-J. Yang, S.-m. Duan, Z.-Q. Xiong, and Y.-Q. Ding Activity-Dependent Development of Callosal Projections in the Somatosensory Cortex J. Neurosci., October 17, 2007; 27(42): 11334 - 11342. [Abstract] [Full Text] [PDF]

				W. Wefelmeyer The Influence of GABA on Dendritic Development In Vivo J. Neurosci., October 3, 2007; 27(40): 10649 - 10650. [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help