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The Journal of Neuroscience, June 27, 2007, 27(26):7070-7080; doi:10.1523/JNEUROSCI.1866-07.2007
 Previous Article
Cellular/Molecular
Dual Regulation of TRPV1 by Phosphoinositides
Viktor Lukacs,1 *
Baskaran Thyagarajan,1 *
Peter Varnai,2
Andras Balla,2
Tamas Balla,2 and
Tibor Rohacs1
1Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey–New Jersey Medical School, Newark, New Jersey 07103, and 2Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
Correspondence should be addressed to Dr. Tibor Rohacs, Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey–New Jersey Medical School, 185 South Orange Avenue, MSB H631, Newark, NJ 07103. Email: tibor.rohacs{at}umdnj.edu
The membrane phospholipid phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2 or PIP2] regulates many ion channels. There are conflicting reports on the effect of PtdIns(4,5)P2 on transient receptor potential vanilloid 1 (TRPV1) channels. We show that in excised patches PtdIns(4,5)P2 and other phosphoinositides activate and the PIP2 scavenger poly-Lys inhibits TRPV1. TRPV1 currents undergo desensitization on exposure to high concentrations of capsaicin in the presence of extracellular Ca2+. We show that in the presence of extracellular Ca2+, capsaicin activates phospholipase C (PLC) in TRPV1-expressing cells, inducing depletion of both PtdIns(4,5)P2 and its precursor PtdIns(4)P (PIP). The PLC inhibitor U73122
[GenBank]
and dialysis of PtdIns(4,5)P2 or PtdIns(4)P through the patch pipette inhibited desensitization of TRPV1, indicating that Ca2+-induced activation of PLC contributes to desensitization of TRPV1 by depletion of PtdIns(4,5)P2 and PtdIns(4)P. Selective conversion of PtdIns(4,5)P2 to PtdIns(4)P by a rapamycin-inducible PIP2 5-phosphatase did not inhibit TRPV1 at high capsaicin concentrations, suggesting a significant role for PtdIns(4)P in maintaining channel activity. Currents induced by low concentrations of capsaicin and moderate heat, however, were potentiated by conversion of PtdIns(4,5)P2 to PtdIns(4)P. Increasing PtdIns(4,5)P2 levels by coexpressing phosphatidylinositol-4-phosphate 5-kinase inhibited TRPV1 at low but not at saturating capsaicin concentrations. These data show that at low capsaicin concentrations and other moderate stimuli, PtdIns(4,5)P2 partially inhibits TRPV1 in a cellular context, but this effect is likely to be indirect, because it is not detectable in excised patches. We conclude that phosphoinositides have both inhibitory and activating effects on TRPV1, resulting in complex and distinct regulation at various stimulation levels.
Key words: TRPV1; TRP channel; phosphoinositides; PIP2; vanilloid; desensitization
Received Dec. 1, 2006;
revised May 23, 2007;
accepted May 24, 2007.
Correspondence should be addressed to Dr. Tibor Rohacs, Department of Pharmacology and Physiology, University of Medicine and Dentistry of New Jersey–New Jersey Medical School, 185 South Orange Avenue, MSB H631, Newark, NJ 07103. Email: tibor.rohacs{at}umdnj.edu
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