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The Journal of Neuroscience, September 26, 2007, 27(39):10404-10413; doi:10.1523/JNEUROSCI.5634-06.2007

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Neurobiology of Disease
Presynaptic Ca²⁺/Calmodulin-Dependent Protein Kinase II Modulates Neurotransmitter Release by Activating BK Channels at Caenorhabditis elegans Neuromuscular Junction

Qiang Liu, ^* Bojun Chen, ^* Qian Ge, ^* and Zhao-Wen Wang

Department of Neuroscience, University of Connecticut Health Center, Farmington, Connecticut 06030

Correspondence should be addressed to Dr. Zhao-Wen Wang, Department of Neuroscience, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-3401. Email: zwwang{at}uchc.edu

Although Ca²⁺/calmodulin-dependent protein kinase II (CaMKII) is enriched at the presynaptic nerve terminal, its role in neurotransmitter release is poorly defined. We assessed the function of presynaptic CaMKII in neurotransmitter release and tested the hypothesis that BK channel is a mediator of presynaptic CaMKII function by analyzing miniature and evoked postsynaptic currents at the Caenorhabditis elegans neuromuscular junction. Both loss-of-function (lf) and gain-of-function (gf) of unc-43, the gene encoding CaMKII, inhibited neurotransmitter release. The inhibitory effect of unc-43(gf) was reversed by mutation or blockade of the BK channel SLO-1. SLO-1 expressed in Xenopus oocytes could be activated by recombinant rat -CaMKII, and this effect of CaMKII was abolished by mutating a threonine residue (T⁴²⁵) at a consensus CaMKII phosphorylation site in the first RCK (regulator of conductance for K⁺) domain of the channel. Expression of slo-1(T⁴²⁵A) in neurons antagonized the inhibitory effect of unc-43(gf) on neurotransmitter release as slo-1(lf) did. The inhibitory effect of unc-43(gf) was not reversed by unc-103(lf), dgk-1(lf), or eat-16(lf), which reportedly suppress behavioral phenotypes of unc-43(gf). These observations suggest that presynaptic CaMKII is a bidirectional modulator of neurotransmitter release, presumably by phosphorylating different molecular targets, and that its negative modulatory effect on the release is mainly mediated by SLO-1 activation.

Key words: BK channel; CaMKII; presynaptic; UNC-103; DGK-1; EAT-16; RCK domain

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Received Dec. 28, 2006; revised July 13, 2007; accepted Aug. 8, 2007.

Correspondence should be addressed to Dr. Zhao-Wen Wang, Department of Neuroscience, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-3401. Email: zwwang{at}uchc.edu

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