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The Journal of Neuroscience, December 19, 2007, 27(51):14007-14011; doi:10.1523/JNEUROSCI.3587-07.2007

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Brief Communications
Synaptic Strength of Individual Spines Correlates with Bound Ca²⁺–Calmodulin-Dependent Kinase II

Brent Asrican,^1 * John Lisman,² and Nikolai Otmakhov^2 *

¹Department of Biology and ²Volen Center For Complex System, Brandeis University, Waltham, Massachusetts 02454

Correspondence should be addressed to Nikolai Otmakhov, Volen Center For Complex System, Brandeis University, 415 South Street, Waltham, MA 02454. Email: Otmakhov{at}brandeis.edu

Both synaptic strength and spine size vary from spine to spine, but are strongly correlated. This gradation is regulated by activity and may underlie information storage. Ca²⁺-calmodulin-dependent kinase II (CaMKII) is critically involved in the regulation of synaptic strength and spine size. The high amount of the kinase in the postsynaptic density has suggested that the kinase has a structural role at synapses. We demonstrated previously that the bound amount of CaMKII in spines persistently increases after induction of long-term potentiation, prompting the hypothesis that this amount may correlate with synaptic strength. To test this hypothesis we combined two recently developed methods, two-photon uncaging of glutamate for determining the EPSC of individual spines (uEPSC) and quantitative microscopy for measuring bound CaMKII in the same spines. We found that under basal conditions the relative bound amount of CaMKII varied over a 10-fold range and positively correlated with the uEPSC. Both the bound amount of CaMKII in spines and uEPSC also positively correlated with spine size. Interestingly, the bound CaMKII fraction (bound/total CaMKII in spines) remained remarkably constant across all spines. The results are consistent with the hypothesis that bound CaMKII serves as a structural organizer of postsynaptic molecules and thereby may be involved in maintaining spine size and synaptic strength.

Key words: dendritic spine; synaptic plasticity; CaMKII; slice culture; fluorescence microscopy; LTP; long-term potentiation; imaging; uncaging

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Received March 27, 2007; revised Oct. 19, 2007; accepted Oct. 25, 2007.

Correspondence should be addressed to Nikolai Otmakhov, Volen Center For Complex System, Brandeis University, 415 South Street, Waltham, MA 02454. Email: Otmakhov{at}brandeis.edu

This article has been cited by other articles:

				Y.-P. Zhang, N. Holbro, and T. G. Oertner Optical induction of plasticity at single synapses reveals input-specific accumulation of {alpha}CaMKII PNAS, August 19, 2008; 105(33): 12039 - 12044. [Abstract] [Full Text] [PDF]

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