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The Journal of Neuroscience, December 19, 2007, 27(51):14171-14178; doi:10.1523/JNEUROSCI.2348-07.2007

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Cellular/Molecular
Differential Trafficking of AMPA and NMDA Receptors during Long-Term Potentiation in Awake Adult Animals

Joanna M. Williams,¹ Diane Guévremont,¹ Sara E. Mason-Parker,² Carthika Luxmanan,¹ Warren P. Tate,³ and Wickliffe C. Abraham²

¹Departments of Anatomy and Structural Biology, ²Psychology, and ³Biochemistry, University of Otago, Dunedin, New Zealand

Correspondence should be addressed to Dr. Joanna M. Williams, Department of Anatomy and Structural Biology, University of Otago, Box 913, Dunedin, New Zealand. Email: joanna.williams{at}stonebow.otago.ac.nz

Despite a wealth of evidence in vitro that AMPA receptors are inserted into the postsynaptic membrane during long-term potentiation (LTP), it remains unclear whether this occurs in vivo at physiological concentrations of receptors. To address the issue of whether native AMPA or NMDA receptors undergo such trafficking during LTP in the adult brain, we examined the synaptic and surface expression of glutamate receptor subunits during the early induction phase of LTP in the dentate gyrus of awake adult rats. Induction of LTP was accompanied by a rapid NMDA receptor-dependent increase in surface expression of glutamate receptor 1–3 (GluR1–3) subunits. However, in the postsynaptic density fraction only GluR1 accumulated. GluR2/3-containing AMPA receptors, in contrast, were targeted exclusively to extrasynaptic sites in a protein synthesis-dependent manner. NMDA receptor subunits exhibited a delayed accumulation, both at the membrane surface and in postsynaptic densities, that was dependent on protein synthesis. These data suggest that trafficking of native GluR1-containing AMPA receptors to synapses is important for early-phase LTP in awake adult animals, and that this increase is followed homeostatically by a protein synthesis-dependent trafficking of NMDA receptors.

Key words: glutamate receptor; long-term potentiation; dentate gyrus; awake animal; synaptoneurosome; postsynaptic density; trafficking

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Received Sept. 6, 2006; revised Sept. 26, 2007; accepted Oct. 30, 2007.

Correspondence should be addressed to Dr. Joanna M. Williams, Department of Anatomy and Structural Biology, University of Otago, Box 913, Dunedin, New Zealand. Email: joanna.williams{at}stonebow.otago.ac.nz

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