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The Journal of Neuroscience, February 7, 2007, 27(6):1405-1410; doi:10.1523/JNEUROSCI.4564-06.2007

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Brief Communications
Presynaptic -Synuclein Aggregates, Not Lewy Bodies, Cause Neurodegeneration in Dementia with Lewy Bodies

Michael L. Kramer and Walter J. Schulz-Schaeffer

Prion and Dementia Research Unit, Institute of Neuropathology, University of Goettingen, 37075 Goettingen, Germany

Correspondence should be addressed to Michael L. Kramer or Walter J. Schulz-Schaeffer at the above address. Email: mkramer{at}med.uni-goettingen.de or Email: wjschulz{at}med.uni-goettingen.de

Lewy bodies, the pathological hallmark of dementia with Lewy bodies (DLB), are large juxtanuclear inclusions of aggregated -synuclein. However, the small number of cortical Lewy bodies relative to the total neuron count does not correlate with the extent of cognitive impairment. In contrast to dopaminergic neurons in Parkinson's disease, nerve cell loss is usually less prevalent in the cortex of DLB, suggesting a different mechanism of neurodegeneration.

Because antibodies used for immunodetection per se do not generally differentiate the aggregated from the physiological and monomeric isoform of -synuclein, we developed the paraffin-embedded tissue (PET) blot and the protein aggregate filtration (PAF) assay for the sensitive and selective detection of -synuclein aggregates in tissue slides and brain homogenates, respectively.

In contrast to common immunohistochemistry, the PET blot detected an enormous number of small -synuclein aggregates, which, in contrast to the few Lewy bodies, may explain the cognitive impairment in DLB. Using the PAF assay, we demonstrate that the absolute majority of -synuclein aggregates are located at presynaptic terminals, suggesting a severe pathological impact on synaptic function. Indeed, parallel to the massive presynaptic accumulation of -synuclein aggregates, we observed significant synaptic pathology with almost complete loss of dendritic spines at the postsynaptic area.

Our results provide strong evidence for a novel concept of neurodegeneration for DLB in which synaptic dysfunction is caused by presynaptic accumulation of -synuclein aggregates. This concept may also be valid for Parkinson's disease.

Key words: -synuclein; dementia with Lewy bodies; Parkinson's disease; dendritic spines; neurodegeneration; Lewy bodies; presynaptic

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Received Oct. 20, 2006; revised Dec. 8, 2006; accepted Dec. 30, 2006.

Correspondence should be addressed to Michael L. Kramer or Walter J. Schulz-Schaeffer at the above address. Email: mkramer{at}med.uni-goettingen.de or Email: wjschulz{at}med.uni-goettingen.de

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