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The Journal of Neuroscience, February 21, 2007, 27(8):2102-2111; doi:10.1523/JNEUROSCI.5436-06.2007

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Cellular/Molecular
Estrogen Mobilizes a Subset of Estrogen Receptor--Immunoreactive Vesicles in Inhibitory Presynaptic Boutons in Hippocampal CA1

Sharron A. Hart, Melissa A. Snyder, Tereza Smejkalova, and Catherine S. Woolley

Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208

Correspondence should be addressed to Dr. Catherine S. Woolley, Northwestern University, 2205 Tech Drive, Evanston, IL 60208. Email: cwoolley{at}northwestern.edu

Although the classical mechanism of estrogen action involves activation of nuclear transcription factor receptors, estrogen also has acute effects on neuronal signaling that occur too rapidly to involve gene expression. These rapid effects are likely to be mediated by extranuclear estrogen receptors associated with the plasma membrane and/or cytoplasmic organelles. Here we used a combination of serial-section electron microscopic immunocytochemistry, immunofluorescence, and Western blotting to show that estrogen receptor- is associated with clusters of vesicles in perisomatic inhibitory boutons in hippocampal CA1 and that estrogen treatment mobilizes these vesicle clusters toward synapses. Estrogen receptor- is present in approximately one-third of perisomatic inhibitory boutons, and specifically in those that express cholecystokinin, not parvalbumin. We also found a high degree of extranuclear estrogen receptor- colocalization with neuropeptide Y. Our results suggest a novel mode of estrogen action in which a subset of vesicles within a specific population of inhibitory boutons responds directly to estrogen by moving toward synapses. The mobilization of these vesicles may influence acute effects of estrogen mediated by estrogen receptor- signaling at inhibitory synapses.

Key words: glutamic acid decarboxylase; GABAergic; cholecystokinin; parvalbumin; neuropeptide Y; serial-section electron microscopy

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Received Dec. 11, 2005; revised Jan. 21, 2007; accepted Jan. 22, 2007.

Correspondence should be addressed to Dr. Catherine S. Woolley, Northwestern University, 2205 Tech Drive, Evanston, IL 60208. Email: cwoolley{at}northwestern.edu

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