QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, March 5, 2008, 28(10):2301-2312; doi:10.1523/JNEUROSCI.5157-07.2008

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Related articles in J. Neurosci. Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Espinosa, J. S. Articles by Luo, L. Search for Related Content PubMed PubMed Citation Articles by Espinosa, J. S. Articles by Luo, L.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Timing Neurogenesis and Differentiation: Insights from Quantitative Clonal Analyses of Cerebellar Granule Cells

J. Sebastian Espinosa and Liqun Luo

Howard Hughes Medical Institute and Department of Biology, Neurosciences Program, Stanford University, Stanford, California 94305

Correspondence should be addressed to Dr. Liqun Luo, Department of Biology, Stanford University, Stanford, CA 94305. Email: lluo{at}stanford.edu

The cerebellum is an excellent model system to study how developmental programs give rise to exquisite neuronal circuits in the adult brain. Here, we describe our findings regarding granule cell neurogenesis and differentiation using the MADM method (mosaic analysis with double markers) in mice. By following the development of individual granule cell clones, we show that (1) granule cell precursors (GCPs) undergo predominantly symmetric division during postnatal development; (2) clonally related granule cells (GCs) exit the cell cycle within a narrow time window and stack their axons in the molecular layer in chronological order from deep to superficial sublayers; and (3) whereas the average GCP proliferation in the external granular layer is progressively slower as development proceeds, there is a rapid expansion of GCPs shortly before clonally related GCs exit the cell cycle. These properties produce GC clones that are distinct, each having a restricted axonal projection, but that are on average similar in cell number. We discuss possible developmental mechanisms and functional implications of these findings.

Key words: cerebellum; cell lineage; granule cells; clonal analysis; neurogenesis; MADM

---

Received Nov. 20, 2007; accepted Jan. 14, 2008.

Correspondence should be addressed to Dr. Liqun Luo, Department of Biology, Stanford University, Stanford, CA 94305. Email: lluo{at}stanford.edu

Related articles in J. Neurosci.:

This Week in The Journal

J. Neurosci. 2008 28: i. [Full Text]  

This article has been cited by other articles:

				R. Desgraz and P. L. Herrera Pancreatic neurogenin 3-expressing cells are unipotent islet precursors Development, November 1, 2009; 136(21): 3567 - 3574. [Abstract] [Full Text] [PDF]

				N. Gajendran, J. P. Kapfhammer, E. Lain, M. Canepari, K. Vogt, W. Wisden, and H. R. Brenner Neuregulin Signaling Is Dispensable for NMDA- and GABAA-Receptor Expression in the Cerebellum In Vivo J. Neurosci., February 25, 2009; 29(8): 2404 - 2413. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help