QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, April 16, 2008, 28(16):4271-4282; doi:10.1523/JNEUROSCI.5566-07.2008

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Murdoch, B. Articles by Roskams, A. J. Search for Related Content PubMed PubMed Citation Articles by Murdoch, B. Articles by Roskams, A. J.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
A Novel Embryonic Nestin-Expressing Radial Glia-Like Progenitor Gives Rise to Zonally Restricted Olfactory and Vomeronasal Neurons

Barbara Murdoch and A. Jane Roskams

Departments of Zoology and Medicine, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3

Correspondence should be addressed to Dr. A. Jane Roskams, Department of Zoology, Life Sciences Centre, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3. Email: roskams{at}zoology.ubc.ca

Persistent neurogenesis is maintained throughout development and adulthood in the mouse olfactory epithelium (OE). Despite this, the identity and origin of different embryonic OE progenitors, their spatiotemporal induction and contribution to patterning during development, has yet to be delineated. Here, we show that the embryonic OE contains a novel nestin-expressing radial glia-like progenitor (RGLP) that is not found in adult OE, which is antigenically distinct from embryonic CNS radial glia. Nestin-cre-mediated lineage tracing with three different reporters reveals that only a subpopulation of nestin-expressing RGLPs activate "CNS-specific" nestin regulatory elements, and produce spatially restricted olfactory receptor neurons (ORNs) in zone 1 of the OE, and vomeronasal receptor neurons restricted to the VR1 zone. This dorsal-medial restriction of transgene-activating cells is also seen in the embryonic OE of Nestin-GFP transgenic mice, in which green fluorescent protein (GFP) is found in a subpopulation of GFP+Mash1+ neuronal progenitors, despite the fact that endogenous Nestin expression is found in RGLPs throughout the OE. Embryonic OE progenitors produce three biologically distinct colony subtypes in vitro, a subpopulation of which include nestin-expressing RGLPs during in vitro colony formation. When generated from Nestin-cre/ZEG mice, neurogenic colonies also produce GFP+Mash1+ progenitors and ORNs. We thus identify a novel neurogenic precursor, the RGLP of the OE and vomeronasal organ (VNO), and provide the first evidence for intrinsic differences in the origin and spatiotemporal potential of distinct progenitors during development of the OE and VNO.

Key words: radial glia; neural progenitors; Nestin-cre; olfactory progenitors; neurogenesis; ensheathing cell

---

Received May 1, 2007; revised Feb. 20, 2008; accepted March 12, 2008.

Correspondence should be addressed to Dr. A. Jane Roskams, Department of Zoology, Life Sciences Centre, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3. Email: roskams{at}zoology.ubc.ca

This article has been cited by other articles:

				M. A. Mena and J. Garcia de Yebenes Glial Cells as Players in Parkinsonism: The "Good," the "Bad," and the "Mysterious" Glia Neuroscientist, December 1, 2008; 14(6): 544 - 560. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help