QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, April 30, 2008, 28(18):4640-4648; doi:10.1523/JNEUROSCI.5486-07.2008

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Hurlock, E. C. Articles by Joho, R. H. PubMed PubMed Citation Articles by Hurlock, E. C. Articles by Joho, R. H.

 Previous Article  |  Next Article 

Cellular/Molecular
Purkinje-Cell-Restricted Restoration of Kv3.3 Function Restores Complex Spikes and Rescues Motor Coordination in Kcnc3 Mutants

Edward C. Hurlock, Anne McMahon, and Rolf H. Joho

Department of Neuroscience, The University of Texas Southwestern Medical Center, Dallas, Texas 75390

Correspondence should be addressed to Dr. Rolf H. Joho, Department of Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9111. Email: rolf.joho{at}utsouthwestern.edu

The fast-activating/deactivating voltage-gated potassium channel Kv3.3 (Kcnc3) is expressed in various neuronal cell types involved in motor function, including cerebellar Purkinje cells. Spinocerebellar ataxia type 13 (SCA13) patients carrying dominant-negative mutations in Kcnc3 and Kcnc3-null mutant mice both display motor incoordination, suggested in mice by increased lateral deviation while ambulating and slips on a narrow beam. Motor skill learning, however, is spared. Mice lacking Kcnc3 also exhibit muscle twitches. In addition to broadened spikes, recordings of Kcnc3-null Purkinje cells revealed fewer spikelets in complex spikes and a lower intraburst frequency. Targeted reexpression of Kv3.3 channels exclusively in Purkinje cells in Kcnc3-null mice as well as in mice also heterozygous for Kv3.1 sufficed to restore simple spike brevity along with normal complex spikes and to rescue specifically coordination. Therefore, spike parameters requiring Kv3.3 function in Purkinje cells are involved in the ataxic null phenotype and motor coordination, but not motor learning.

Key words: K channels; burst firing; motor dysfunction; cerebellum; Purkinje cell; complex spike

---

Received Dec. 12, 2007; revised March 14, 2008; accepted March 17, 2008.

Correspondence should be addressed to Dr. Rolf H. Joho, Department of Neuroscience, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9111. Email: rolf.joho{at}utsouthwestern.edu

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help