QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, June 4, 2008, 28(23):5910-5919; doi:10.1523/JNEUROSCI.0632-08.2008

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (10) Citing Articles via Google Scholar Google Scholar Articles by Lee, R. Articles by Mason, C. A. Search for Related Content PubMed PubMed Citation Articles by Lee, R. Articles by Mason, C. A.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Zic2 Regulates Retinal Ganglion Cell Axon Avoidance of ephrinB2 through Inducing Expression of the Guidance Receptor EphB1

Ramee Lee, Timothy J. Petros, and Carol A. Mason

Departments of Pathology and Cell Biology, and Neuroscience, Columbia University, College of Physicians and Surgeons, New York, New York 10032

Correspondence should be addressed to Ramee Lee, Departments of Pathology and Cell Biology, and Neuroscience, Columbia University, College of Physicians and Surgeons, 14-509 P&S Building, 630 West 168th Street, New York, NY 10032. Email: rl2159{at}columbia.edu

The navigation of retinal axons to ipsilateral and contralateral targets in the brain depends on the decision to cross or avoid the midline at the optic chiasm, a critical guidance maneuver that establishes the binocular visual pathway. Previous work has identified a specific guidance receptor, EphB1, that mediates the repulsion of uncrossed axons away from its ligand, ephrinB2, at the optic chiasm midline (Williams et al., 2003), and a transcription factor Zic2, that, like EphB1, is required for formation of the ipsilateral retinal projection (Herrera et al., 2003). Although the reported similarities in localization implicated that Zic2 regulates EphB1 (Herrera et al., 2003; Williams et al., 2003; Pak et al., 2004), whether Zic2 drives expression of EphB1 protein has not been elucidated. Here we show that EphB1 protein is expressed in the growth cones of axons from ventrotemporal (VT) retina that project ipsilaterally and that repulsion by ephrinB2 is determined by the presence of this receptor on growth cones. Moreover, ectopic delivery of Zic2 into explants from non-VT retina induces expression of EphB1 mRNA and protein. The upregulated EphB1 receptor protein is localized to growth cones and is functional, because it is sufficient to change retinal ganglion cell axon behavior from extension onto, to avoidance of, ephrinB2 substrates. Our results demonstrate that Zic2 upregulates EphB1 expression and define a link between a transcription factor and expression of a guidance receptor protein essential for axon guidance at the vertebrate midline.

Key words: retinal ganglion cell; optic chiasm; midline; ipsilateral retinal projection; guidance factor; intermediate target; axon guidance

---

Received Feb. 12, 2008; revised April 4, 2008; accepted April 22, 2008.

Correspondence should be addressed to Ramee Lee, Departments of Pathology and Cell Biology, and Neuroscience, Columbia University, College of Physicians and Surgeons, 14-509 P&S Building, 630 West 168th Street, New York, NY 10032. Email: rl2159{at}columbia.edu

This article has been cited by other articles:

				A. Rebsam, T. J. Petros, and C. A. Mason Switching Retinogeniculate Axon Laterality Leads to Normal Targeting but Abnormal Eye-Specific Segregation That Is Activity Dependent J. Neurosci., November 25, 2009; 29(47): 14855 - 14863. [Abstract] [Full Text] [PDF]

				N. A. Sanek, A. A. Taylor, M. K. Nyholm, and Y. Grinblat Zebrafish zic2a patterns the forebrain through modulation of Hedgehog-activated gene expression Development, November 15, 2009; 136(22): 3791 - 3800. [Abstract] [Full Text] [PDF]

				S. Nobrega-Pereira and O. Marin Transcriptional Control of Neuronal Migration in the Developing Mouse Brain Cereb Cortex, July 1, 2009; 19(suppl_1): i107 - i113. [Abstract] [Full Text] [PDF]

				M. K. Nyholm, S. Abdelilah-Seyfried, and Y. Grinblat A novel genetic mechanism regulates dorsolateral hinge-point formation during zebrafish cranial neurulation J. Cell Sci., June 15, 2009; 122(12): 2137 - 2148. [Abstract] [Full Text] [PDF]

				M. M. Corty, B. J. Matthews, and W. B. Grueber Molecules and mechanisms of dendrite development in Drosophila Development, April 1, 2009; 136(7): 1049 - 1061. [Abstract] [Full Text] [PDF]

				T. J. Petros, B. R. Shrestha, and C. Mason Specificity and Sufficiency of EphB1 in Driving the Ipsilateral Retinal Projection J. Neurosci., March 18, 2009; 29(11): 3463 - 3474. [Abstract] [Full Text] [PDF]

				N. M. Tian, T. Pratt, and D. J. Price Foxg1 regulates retinal axon pathfinding by repressing an ipsilateral program in nasal retina and by causing optic chiasm cells to exert a net axonal growth-promoting activity Development, December 15, 2008; 135(24): 4081 - 4089. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help