QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, August 13, 2008, 28(33):8294-8305; doi:10.1523/JNEUROSCI.2010-08.2008

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (7) Citing Articles via Google Scholar Google Scholar Articles by Dugas, J. C. Articles by Barres, B. A. Search for Related Content PubMed PubMed Citation Articles by Dugas, J. C. Articles by Barres, B. A.

 Previous Article  |  Next Article 

Cellular/Molecular
A Novel Purification Method for CNS Projection Neurons Leads to the Identification of Brain Vascular Cells as a Source of Trophic Support for Corticospinal Motor Neurons

Jason C. Dugas, ^* Wim Mandemakers, ^* Madolyn Rogers, Adiljan Ibrahim, Richard Daneman, and Ben A. Barres

Department of Neurobiology, Stanford University School of Medicine, Stanford, California 94305-5125

Correspondence should be addressed to Dr. Jason C. Dugas, Department of Neurobiology, Stanford University School of Medicine, Fairchild Building, Room D205, 299 Campus Drive, Stanford, CA 94305-5125. Email: jcdugas{at}alum.mit.edu

One of the difficulties in studying cellular interactions in the CNS is the lack of effective methods to purify specific neuronal populations of interest. We report the development of a novel purification scheme, cholera toxin β (CTB) immunopanning, in which a particular CNS neuron population is selectively labeled via retrograde axonal transport of the cell-surface epitope CTB, and then purified via immobilization with anti-CTB antibody. We have demonstrated the usefulness and versatility of this method by purifying both retinal ganglion cells and corticospinal motor neurons (CSMNs). Genomic expression analyses of purified CSMNs revealed that they express significant levels of many receptors for growth factors produced by brain endothelial cells; three of these factors, CXCL12, pleiotrophin, and IGF2 significantly enhanced purified CSMN survival, similar to previously characterized CSMN trophic factors BDNF and IGF1. In addition, endothelial cell conditioned medium significantly promoted CSMN neurite outgrowth. These findings demonstrate a useful method for the purification of several different types of CNS projection neurons, which in principle should work in many mammalian species, and provide evidence that endothelial-derived factors may represent an overlooked source of trophic support for neurons in the brain.

Key words: corticospinal motoneurons; immunopanning; endothelial cells; IGF2; CXCL12; SDF1; pleiotrophin

---

Received May 1, 2008; revised June 23, 2008; accepted June 30, 2008.

Correspondence should be addressed to Dr. Jason C. Dugas, Department of Neurobiology, Stanford University School of Medicine, Fairchild Building, Room D205, 299 Campus Drive, Stanford, CA 94305-5125. Email: jcdugas{at}alum.mit.edu

This article has been cited by other articles:

				K. Arai and E. H. Lo An Oligovascular Niche: Cerebral Endothelial Cells Promote the Survival and Proliferation of Oligodendrocyte Precursor Cells J. Neurosci., April 8, 2009; 29(14): 4351 - 4355. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help