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The Journal of Neuroscience, September 10, 2008, 28(37):9309-9318; doi:10.1523/JNEUROSCI.1905-08.2008

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Behavioral/Systems/Cognitive
Spatiotemporal Profiles of Field Potentials in Mouse Superior Colliculus Analyzed by Multichannel Recording

Penphimon Phongphanphanee,^1,2 Katsuyuki Kaneda,^1,2 and Tadashi Isa^1,2,3

¹Department of Developmental Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, Japan, ²The Graduate University for Advanced Studies (Sokendai), Hayama 240-0193, Japan, and ³The Core Research for Evolutionary Science and Technology, Japan, Science and Technology Agency, Kawaguchi 332-0012, Japan

Correspondence should be addressed to Dr. Tadashi Isa, Department of Developmental Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, Japan. Email: tisa{at}nips.ac.jp

The onset and vector of orienting behaviors, such as saccades, are controlled by commands that descend from a population of neurons in deep layers of the superior colliculus (dSC). In this study, to characterize the role of the collicular local circuitry that generates the spatiotemporal pattern of command activity in the dSC neuronal population, responses evoked by single-pulse electrical stimulation in superficial layers of the superior colliculus (sSC) were analyzed by a 64-channel field potential recording system (planar electrode, 8 x 8 pattern; 150 µm interelectrode spacing) in slices obtained from 16- to 22-d-old mice. A negative field potential with short latency and short duration spatially restricted to the recording sites in sSC was evoked adjacent to the stimulation site. After bath application of 10 µM bicuculline, the same stimulus induced a large negative field response with long duration that spread from sSC to dSC. The dSC potential initially showed a positive response, presumably because of reversal of the negative potential that originated in sSC, and then a long negative response that spread horizontally as far as 1 mm. These responses disappeared after application of an NMDA receptor antagonist, 2-amino-5-phosphonovelarate, indicating that NMDA receptors have an important role in the generation of these responses. Simultaneous whole-cell patch-clamp recordings showed that the long-lasting negative field potentials corresponded to the depolarization accompanying burst spike activity of SC neurons. The present study revealed an extensive excitatory network in the dSC that may contribute to the generation of activity by a large population of neurons that discharge before a saccade.

Key words: field potential; patch clamp; signal propagation; slice; burst; saccade

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Received April 30, 2008; revised July 18, 2008; accepted Aug. 14, 2008.

Correspondence should be addressed to Dr. Tadashi Isa, Department of Developmental Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, Japan. Email: tisa{at}nips.ac.jp

This article has been cited by other articles:

				T. Isa and W. C. Hall Exploring the Superior Colliculus In Vitro J Neurophysiol, November 1, 2009; 102(5): 2581 - 2593. [Abstract] [Full Text] [PDF]

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