QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, October 8, 2008, 28(41):10257-10271; doi:10.1523/JNEUROSCI.2471-08.2008

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Related articles in J. Neurosci. Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Bushlin, I. Articles by Yao, P. J. Search for Related Content PubMed PubMed Citation Articles by Bushlin, I. Articles by Yao, P. J. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein UniGene

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Clathrin Assembly Protein AP180 and CALM Differentially Control Axogenesis and Dendrite Outgrowth in Embryonic Hippocampal Neurons

Ittai Bushlin,¹ Ronald S. Petralia,² Fangbai Wu,¹ Asaff Harel,¹ Mohamed R. Mughal,¹ Mark P. Mattson,¹ and Pamela J. Yao¹

¹Laboratory of Neurosciences, National Institute on Aging–National Institutes of Health, Baltimore, Maryland 21224, and ²Laboratory of Neurochemistry, National Institute on Deafness and Other Communication Disorders–National Institutes of Health, Bethesda, Maryland 20892

Correspondence should be addressed to Dr. Pamela J. Yao, Laboratory of Neurosciences, The National Institute on Aging–National Institutes of Health Biomedical Research Center, 251 Bayview Boulevard, Baltimore, MD 21224. Email: yaopa{at}grc.nia.nih.gov

Emerging data suggest that, much like epithelial cells, the polarized growth of neurons requires both the secretory and endocytic pathways. The clathrin assembly proteins AP180 and CALM (clathrin assembly lymphoid myeloid protein) are known to be involved in clathrin-mediated endocytosis, but their roles in mammalian neurons and, in particular, in developmental processes before synaptogenesis are unknown. Here we provide evidence that AP180 and CALM play critical roles in establishing the polarity and controlling the growth of axons and dendrites in embryonic hippocampal neurons. Knockdown of AP180 primarily impairs axonal development, whereas reducing CALM levels results in dendritic dystrophy. Conversely, neurons that overexpress AP180 or CALM generate multiple axons. Ultrastructural analysis shows that CALM affiliates with a wider range of intracellular trafficking organelles than does AP180. Functional analysis shows that endocytosis is reduced in both AP180-deficient and CALM-deficient neurons. Additionally, CALM-deficient neurons show disrupted secretory transport. Our data demonstrate previously unknown functions for AP180 and CALM in intracellular trafficking that are essential in the growth of neurons.

Key words: AP180; CALM; trafficking; neuron; polarity; development

---

Received June 1, 2008; revised July 29, 2008; accepted Aug. 18, 2008.

Correspondence should be addressed to Dr. Pamela J. Yao, Laboratory of Neurosciences, The National Institute on Aging–National Institutes of Health Biomedical Research Center, 251 Bayview Boulevard, Baltimore, MD 21224. Email: yaopa{at}grc.nia.nih.gov

Related articles in J. Neurosci.:

This Week in The Journal

J. Neurosci. 2008 28: i. [Full Text]  

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help