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The Journal of Neuroscience, October 8, 2008, 28(41):10434-10442; doi:10.1523/JNEUROSCI.2831-08.2008

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Development/Plasticity/Repair
Progeny of Olig2-Expressing Progenitors in the Gray and White Matter of the Adult Mouse Cerebral Cortex

Leda Dimou,¹ Christiane Simon,¹ Frank Kirchhoff,² Hirohide Takebayashi,³ and Magdalena Götz^1,4,5

¹Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University Munich, 80336 Munich, Germany, ²Department of Neurogenetics, Max Planck Institute of Experimental Medicine, 37075 Goettingen, Germany, ³Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki 444-8585, Japan, ⁴Institute for Stem Cell Research, HelmholtzZentrum München, 85764 Neuherberg, Germany, and ⁵Center for Integrated Protein Science Munich, Ludwig-Maximilians University, 81377 Munich, Germany

Correspondence should be addressed to either of the following: Leda Dimou, Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University Munich, 80336 Munich, Germany, Email: leda.dimou{at}lrz.uni-muenchen.de; or Magdalena Götz, Institute for Stem Cell Research, HelmholtzZentrum München, 85764 Neuherberg, Germany, Email: magdalena.goetz{at}helmholtz-muenchen.de

Despite their abundance, still little is known about the rather frequent, constantly proliferating progenitors spread throughout the adult mouse brain parenchyma. The majority of these progenitors express the basic-helix-loop-helix transcription factor Olig2, and their number further increases after injury. Here, we examine the progeny of this progenitor population by genetic fate mapping using tamoxifen-inducible Cre-recombination in the Olig2 locus to turn on permanent reporter gene expression in the adult brain. Consistent with Olig2 expression in proliferating NG2⁺ progenitors, most reporter⁺ cells seen shortly after initiating recombination at adult stages incorporated BrdU and contained the proteoglycan NG2 in both the gray (GM) and the white matter (WM) of the cerebral cortex. However, at longer time points after induction, we observed profound differences in the identity of reporter⁺ cells in the WM and GM. Whereas most of the Olig2⁺ progenitors had generated mature, myelinating oligodendrocytes in the WM, hardly any reporter⁺ cells showing mature oligodendrocyte characteristics were detectable even up to 6 months after recombination in the GM. In the GM, most reporter⁺ cells remained NG2⁺, even after injury, but stopped proliferating rather soon after recombination. Thus, our results demonstrate the continuous generation of mature, myelinating oligodendrocytes in the WM, whereas cells in the GM generated mostly postmitotic NG2⁺ glia.

Key words: oligodendrocyte progenitors; NG2; myelination; stab wound injury; forebrain; lineage analysis

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Received June 19, 2008; revised Aug. 29, 2008; accepted Sept. 2, 2008.

Correspondence should be addressed to either of the following: Leda Dimou, Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University Munich, 80336 Munich, Germany, Email: leda.dimou{at}lrz.uni-muenchen.de; or Magdalena Götz, Institute for Stem Cell Research, HelmholtzZentrum München, 85764 Neuherberg, Germany, Email: magdalena.goetz{at}helmholtz-muenchen.de

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