QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, November 12, 2008, 28(46):11970-11979; doi:10.1523/JNEUROSCI.3724-08.2008

This Article Full Text Full Text (PDF) Supplemental Data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Li, P. Articles by Murphy, T. H. Search for Related Content PubMed PubMed Citation Articles by Li, P. Articles by Murphy, T. H.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Two-Photon Imaging during Prolonged Middle Cerebral Artery Occlusion in Mice Reveals Recovery of Dendritic Structure after Reperfusion

Ping Li¹ and Timothy H. Murphy^1,2,3

¹Kinsmen Laboratory, Department of Psychiatry, ²Brain Research Center, and ³Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3

Correspondence should be addressed to Dr. Timothy H. Murphy, Department of Psychiatry, University of British Columbia, 4N1-2255 Wesbrook Mall, Vancouver, British Columbia, Canada V6T 1Z3. Email: thmurphy{at}interchange.ubc.ca

Filament occlusion of the middle cerebral artery (MCA) is a well accepted animal model of focal ischemia. Advantages of the model are relatively long occlusion times and a large penumbra region that simulates aspects of human stroke. Here, we use two-photon and confocal microscopy in combination with regional measurement of blood flow using laser speckle to assess the spatial relationship between the borders of the MCA ischemic territory and loss of dendrite structure, as well as the effect of reperfusion on dendritic damage in adult YFP (yellow fluorescent protein) and GFP (green fluorescent protein) C57BL/6 transgenic mice with fluorescent (predominantly layer 5) neurons. By examining the spatial extent of dendritic damage, we determined that 60 min of MCA occlusion produced a core with severe structural damage that did not recover after reperfusion (begins 3.8 mm lateral to midline), a reversibly damaged area up to 0.6 mm medial to the core that recovered after reperfusion (penumbra), and a relatively structurally intact area (1 mm wide; medial penumbra) with hypoperfusion. Loss of structure was preceded by a single ischemic depolarization 122.1 ± 10.2 s after occlusion onset. Reperfusion of animals after 60 min of ischemia was not associated with exacerbation of damage (reperfusion injury) and resulted in a significant restoration of blebbed dendritic structure, but only within 0.6 mm lateral of the dendritic damage structural border. In summary, we find that recovery of dendritic structure can occur after reperfusion after even 60 min of ischemia, but is likely restricted to a relatively small penumbra region with partial blood flow or oxygenation.

Key words: stroke; imaging; dendritic spine; blood flow; ischemia; dendrite

---

Received Aug. 6, 2008; revised Sept. 24, 2008; accepted Sept. 29, 2008.

Correspondence should be addressed to Dr. Timothy H. Murphy, Department of Psychiatry, University of British Columbia, 4N1-2255 Wesbrook Mall, Vancouver, British Columbia, Canada V6T 1Z3. Email: thmurphy{at}interchange.ubc.ca

This article has been cited by other articles:

				N. Jaenisch, O. W. Witte, and C. Frahm Downregulation of Potassium Chloride Cotransporter KCC2 After Transient Focal Cerebral Ischemia Stroke, March 1, 2010; 41(3): e151 - e159. [Abstract] [Full Text] [PDF]

				R. R. Liu and T. H. Murphy Reversible Cyclosporin A-sensitive Mitochondrial Depolarization Occurs within Minutes of Stroke Onset in Mouse Somatosensory Cortex in Vivo: A TWO-PHOTON IMAGING STUDY J. Biol. Chem., December 25, 2009; 284(52): 36109 - 36117. [Abstract] [Full Text] [PDF]

				I. R. Winship and T. H. Murphy Remapping the Somatosensory Cortex after Stroke: Insight from Imaging the Synapse to Network Neuroscientist, October 1, 2009; 15(5): 507 - 524. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help