QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (80) Citing Articles via Google Scholar Google Scholar Articles by Goldberg, W. J. Articles by Barrett, J. N. Search for Related Content PubMed PubMed Citation Articles by Goldberg, W. J. Articles by Barrett, J. N.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 6, 3144-3151, Copyright © 1986 by Society for Neuroscience

ARTICLE

Effects of ischemia-like conditions on cultured neurons: protection by low Na+, low Ca2+ solutions

WJ Goldberg, RM Kadingo and JN Barrett

An in vitro system was used to mimic several aspects of ischemia, including low oxygen pressure, low nutrient levels, and the accumulation of cellular products thought to contribute to damage during ischemia. We replaced normal culture medium from 3-week-old basal ganglia cultures with oxygen-depleted, nutrient-deficient medium. After incubation in an atmosphere of 94% N2, 6% CO2 for 5 hr at 37 degrees C, the cultures were returned to normal medium. After a 24 hr recovery period, cell viability was assessed in terms of cell number, electrophysiological properties, and immunohistochemical markers. When the medium used during the ischemic period was a normal balanced salt solution, more than 70% of the cells were damaged by the low-oxygen, low-glucose stress. Loss of cell processes and cell swelling were the most evident signs of damage. The majority of the cells remaining viable were astrocytes. Neuronal damage was observed only when both glucose and oxygen were deficient. Some damage was evident even at oxygen tensions of 60 mm Hg when glucose was absent from the medium; much more extensive damage was observed at tensions below 1.0 mm Hg. Lowering both extracellular sodium and calcium resulted in more than a 2-fold increase in survival (70 vs 28%). These results indicate that damage to neurons during conditions of extreme energy deprivation such as ischemia may be mediated by the influx of calcium and/or sodium.

This article has been cited by other articles:

				P. Lipton Ischemic Cell Death in Brain Neurons Physiol Rev, October 1, 1999; 79(4): 1431 - 1568. [Abstract] [Full Text] [PDF]

				E. Guatteo, N. B. Mercuri, G. Bernardi, and T. Knopfel Intracellular Sodium and Calcium Homeostasis During Hypoxia in Dopamine Neurons of Rat Substantia Nigra Pars Compacta J Neurophysiol, November 1, 1998; 80(5): 2237 - 2243. [Abstract] [Full Text] [PDF]

				J. Hill Lucas, D. G. Emery, and L. J. Rosenberg REVIEW {blacksquare} : Physical Injury of Neurons: Important Roles for Sodium and Chloride Ions Neuroscientist, March 1, 1997; 3(2): 89 - 101. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help