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Journal of Neuroscience, Vol 6, 3640-3654, Copyright © 1986 by Society for Neuroscience
Coexistence of galanin-like immunoreactivity with catecholamines, 5- hydroxytryptamine, GABA and neuropeptides in the rat CNS
T Melander, T Hokfelt, A Rokaeus, AC Cuello, WH Oertel, A Verhofstad and M Goldstein
The coexistence of galanin (GAL)-like immunoreactivity (LI) with markers
for catecholamines, 5-hydroxytryptamine (5-HT), GABA, or some neuropeptides
was mapped in the rat CNS by using adjacent sections, as well as by
elution-restaining and double-labeling immunocytochemistry. Many instances
of coexistence were observed, but there were also numerous GAL-positive
cell body populations displaying distributions similar to those of these
markers but without apparent coexistence. In the hypothalamic arcuate
nucleus GAL-LI was found in a large proportion of tyrosine hydroxylase
(TH)-positive cell bodies (A12 cells), both in the dorsomedial and
ventrolateral subdivisions, with a higher number in the latter. GAL-LI
coexisted in glutamic acid decarboxylase (GAD)- positive somata in the
posterior aspects of the arcuate nucleus and at all rostrocaudal levels in
fibers in the external layer of the median eminence. In the anterior
hypothalamus, a large population of the cells of the parvocellular and
magnocellular paraventricular nuclei contained both GAL-LI and
vasopressin-LI. Moreover, somata containing both GAD- and GAL-LI were seen
lateral to the mammillary recess in the tuberal and caudal magnocellular
nuclei. Some of the neurons of the caudal group were shown to project to
the occipital cortex using combined retrograde tracing and
immunofluorescence. With regard to mesencephalic and medullary
catecholamine neurons, GAL-LI coexisted in a large proportion of the
noradrenergic locus coeruleus somata (A6 cell group) and in the A4 group
dorsolateral to the fourth ventricle, as well as in the caudal parts of the
A2 group in the dorsal vagal complex. However, in more rostral parts of the
latter, especially in the medial subdivision of the solitary tract nucleus,
a very large population of GAL-IR small cell bodies was seen intermingling
with catecholamine neurons, but they did not contain TH-LI. Furthermore,
GAL-IR cell bodies coextensive with, but not coexisting in, TH-IR somata
were seen in the C1 (epinephrine) horea in the ventrolateral medulla at the
level of area postrema and in the most rostral aspects of the C1 group.
Finally, 5-HT-positive cell bodies of the mesencephalic and medullary raphe
nuclei and a subpopulation of coarse 5-HT nerve fibers in the hippocampus
co-contained GAL-LI. The present results demonstrate that a GAL-like
peptide is present in many systems containing other neuroactive compounds,
including dopamine, norepinephrine, 5-HT, GABA, and vasopressin.(ABSTRACT
TRUNCATED AT 400 WORDS)
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