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Journal of Neuroscience, Vol 6, 650-660, Copyright © 1986 by Society for Neuroscience
Expression of neurofilament subunits in neurons of the central and peripheral nervous system: an immunohistochemical study with monoclonal antibodies
JQ Trojanowski, N Walkenstein and VM Lee
The extent to which all neurofilament (NF) subunits (NF68, NF150, NF200)
are expressed by different populations of mature CNS and PNS neurons is
controversial. We addressed this issue in immunohistochemical studies of
mature bovine tissues using monoclonal antibodies specific for each bovine
NF subunit. All three NF subunits were detected in the perikarya and
neurites of both CNS and PNS neurons; they were seen in nearly all PNS
neuronal perikarya, and in all identifiable CNS and PNS axons. Most, but
not all, CNS neuronal perikarya contained each of these NF antigens. CNS
neurons devoid of immunodetectable NF antigens were generally small. The
presence of low levels of NF antigens in neurons with scant perikaryal
cytoplasm may account for the apparent absence of NF immunoreactivity in
some classes of neurons, although other explanations, such as
microheterogeneity among NF proteins, could account for this finding. NF
antigens were also seen in some cells of the diffuse neuroendocrine system
(adrenal chromaffin cells and cells of the pars distalis and pars
intermedia), but not in other cell types. We suggest that the expression of
all three NF subunits is a common feature of CNS and PNS neurons and their
processes, and of some cells of the diffuse neuroendocrine system. These
findings have implications for hypotheses concerning the structure and
function of the intermediate filaments of neurons, and for hypotheses
concerning neurodegenerative diseases involving NF proteins.
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