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Journal of Neuroscience, Vol 6, 1241-1251, Copyright © 1986 by Society for Neuroscience
The participation of a putative cell surface receptor for laminin and fibronectin in peripheral neurite extension
D Bozyczko and AF Horwitz
We have used the CSAT (cell substrate attachment) monoclonal antibody
(Mab), which is directed against a putative laminin and fibronectin
receptor, to examine its role in the adhesive phenomena of neurons. This
antibody was previously found to disturb the adhesion of several classes of
fibroblasts and muscle. Here we report its effects upon neuronal-substrate
adhesion. Two sources of neurons were investigated-- the dorsal root and
ciliary ganglia. Both responded similarly. Neurons plated in the presence
of the CSAT Mab did not adhere to the substratum and process formation was
inhibited completely for at least 24-48 hr. In explant cultures, when
neurons were first allowed to extend processes prior to addition of the
CSAT Mab, the results depended on the particular substrate. With some
substrates, the neurites bundled and detached from the substratum; with
others, they retracted and regrew to form large fascicles or bundles of
processes. In dissociated cultures that already had extended processes,
neurites fasciculated and cell bodies aggregated in response to the
presence of the CSAT Mab. The magnitude of this response varied, depending
upon the substrate. The antigen was localized, using immunofluorescence, on
neuronal cell bodies, axons, and growth cones. This distribution correlated
with its biological effects on all parts of the neuron. The antigen was
isolated from neuronal cultures by immunoaffinity purification. It migrated
in the molecular weight range of 140 kDa on reducing SDS-PAGE. This antigen
is very similar to that isolated from fibroblasts, which is an integral
membrane glycoprotein complex. The data presented implicate the
participation of the CSAT antigen in neurite extension and fasciculation.
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