 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 6, 1358-1364, Copyright © 1986 by Society for Neuroscience
The glycine receptor deficiency of the mutant mouse spastic: evidence for normal glycine receptor structure and localization
CM Becker, I Hermans-Borgmeyer, B Schmitt and H Betz
Homozygotes of the mutant mouse spastic exhibit reduced binding of 3H-
strychnine to homogenates from various regions of the CNS compared with
unaffected littermates (White and Heller, 1982). Here we report evidence
that the spastic mutation coincides with a reduced concentration and an
unaltered structure of the glycine receptor in spinal cord. Scatchard
analysis of 3H-strychnine binding revealed a single binding site with a
Bmax of 267 +/- 62 fmol/mg protein for spastic and of 864 +/- 220 fmol/mg
protein for control mice; no difference was found for the corresponding KD
values. Also Ki values of glycine for 3H-strychnine binding and
displacement of 3H-strychnine by beta-alanine and taurine were
indistinguishable for both preparations. Photoaffinity labeling of synaptic
membranes with 3H-strychnine identified an Mr = 48,000 polypeptide in both
control and spastic mouse membranes. Tryptic digestion of these membranes
produced radiolabeled peptide fragments of identical molecular weights,
suggesting that the proteolytic cleavage sites around the antagonist
binding site are conserved in the mutant glycine receptor protein. Glycine
receptors from both control and mutant mice were purified by affinity
chromatography on aminostrychnine agarose. SDS/PAGE revealed three
polypeptides of Mr = 48,000, 58,000, and 93,000 in both receptor
preparations. Monoclonal antibodies directed against different subunits of
the glycine receptor were applied to an enzyme-linked immunosorbent assay.
The same pattern of immunoreactivity was obtained for glycine receptor from
spinal cord of spastic homozygotes, control mice, and rats, suggesting
conservation of the antigenic epitopes in the mutant receptor.(ABSTRACT
TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
|
|
 
N. Tian
Synaptic activity, visual experience and the maturation of retinal synaptic circuitry
J. Physiol.,
September 15, 2008;
586(18):
4347 - 4355.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Graham, P. R. Schofield, P. Sah, T. W. Margrie, and R. J. Callister
Distinct physiological mechanisms underlie altered glycinergic synaptic transmission in the murine mutants spastic, spasmodic, and oscillator.
J. Neurosci.,
May 3, 2006;
26(18):
4880 - 4890.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Becker, J. von Wegerer, J. Schenkel, H.-U. Zeilhofer, D. Swandulla, and H. Weiher
Disease-Specific Human Glycine Receptor alpha 1 Subunit Causes Hyperekplexia Phenotype and Impaired Glycine- and GABAA-Receptor Transmission in Transgenic Mice
J. Neurosci.,
April 1, 2002;
22(7):
2505 - 2512.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik
Molecular Structure and Physiological Function of Chloride Channels
Physiol Rev,
April 1, 2002;
82(2):
503 - 568.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. U. Zeilhofer, U. M. Selbach, H. Guhring, K. Erb, and S. Ahmadi
Selective Suppression of Inhibitory Synaptic Transmission by Nocistatin in the Rat Spinal Cord Dorsal Horn
J. Neurosci.,
July 1, 2000;
20(13):
4922 - 4929.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kneussel, J. H. Brandstatter, B. Laube, S. Stahl, U. Muller, and H. Betz
Loss of Postsynaptic GABAA Receptor Clustering in Gephyrin-Deficient Mice
J. Neurosci.,
November 1, 1999;
19(21):
9289 - 9297.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U Monani and A H Burghes
Structure of the human alpha 2 subunit gene of the glycine receptor--use of vectorette and Alu-exon PCR.
Genome Res.,
December 1, 1996;
6(12):
1200 - 1206.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Kirsch and S. Kroger
{blacksquare} REVIEW : Postsynaptic Anchoring of Receptors: A Cellular Approach to Neuronal and Muscular Sensitivity
Neuroscientist,
March 1, 1996;
2(2):
100 - 108.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C.-M. Becker
Review : Glycine Receptors: Molecular Heterogeneity and Implications for Disease
Neuroscientist,
May 1, 1995;
1(3):
130 - 141.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. Gundlach, P. Dodd, C. Grabara, W. Watson, G. Johnston, P. Harper, J. Dennis, and P. Healy
Deficit of spinal cord glycine/strychnine receptors in inherited myoclonus of Poll Hereford calves
Science,
September 30, 1988;
241(4874):
1807 - 1810.
[Abstract]
[PDF]
|
|
|
|
