QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brenner, T. Articles by Silberberg, D. H. Search for Related Content PubMed PubMed Citation Articles by Brenner, T. Articles by Silberberg, D. H.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 6, 1925-1933, Copyright © 1986 by Society for Neuroscience

ARTICLE

Astrocytes, oligodendrocytes, and Schwann cells share a common antigenic determinant that cross-reacts with myelin basic protein: identification with monoclonal antibody

T Brenner, RP Lisak, A Rostami, DE Pleasure and DH Silberberg

We have produced a monoclonal antibody against myelin basic protein that reacts with astrocytes, oligodendrocytes, and Schwann cells. This antibody was generated by fusion of mouse myeloma cells with spleen cells from BALB/c mice immunized with delipidated white matter from adult rat corpus callosum. The antibody was characterized via solid- phase radioimmunoassay, immunoblot of SDS-PAGE, and by indirect immunofluorescence staining of monolayer cultures containing oligodendrocytes, astrocytes, and Schwann cells. Myelin basic protein (MBP) was shown previously to be present only in myelin producing cells in CNS and PNS (oligodendroglia and Schwann cells) and not in astrocytes. The binding of this monoclonal antibody to all 3 cell types suggests that these cells share a common epitope. This epitope may be related to a common progenitor cell.

This article has been cited by other articles:

				D. Shemi, A. Azab, and J. Kaplanski The effect of high and low ambient temperatures on PGE2 and TNF-{alpha} production by rat glial brain cultures Innate Immunity, June 1, 2003; 9(3): 165 - 168. [Abstract] [PDF]

				D. Shemi, A.N. Azab, and J. Kaplanski Time-dependent effect of LPS on PGE2 and TNF-{alpha} production by rat glial brain culture: influence of COX and cytokine inhibitors Innate Immunity, October 1, 2000; 6(5): 377 - 381. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help