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Journal of Neuroscience, Vol 7, 3293-3299, Copyright © 1987 by Society for Neuroscience
Tight junctions of brain endothelium in vitro are enhanced by astroglia
JH Tao-Cheng, Z Nagy and MW Brightman
Laboratory of Neurobiology, NIH/NINCDS, Bethesda, Maryland 20892.
The belts of endothelial tight junctions, which impede diffusion between
blood and brain, were reduced to fragmentary, small junctions in
subcultured brain endothelium. When cocultured with the capillaries'
nearest neighbor, the astrocytes, these endothelial tight junctions were
enhanced in length, width, and complexity, as seen by en face views of the
cell membranes with freeze-fracture electron microscopy. Gap junctions,
common in brain endothelium in vitro but absent in mature brain capillaries
in vivo, were markedly diminished in area from among the enhanced tight
junctions of the cocultures. Thus, astrocytes in vitro play a role in the
formation, extent, and configuration of the junctional complexes in brain
endothelium, whose diffusion barrier may likewise be influenced by
astrocytes in vivo.
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