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Journal of Neuroscience, Vol 7, 1207-1214, Copyright © 1987 by Society for Neuroscience


ARTICLE

Fluorescent probes that stain living nerve terminals

L Magrassi, D Purves and JW Lichtman

We have evaluated the efficacy of 18 cationic mitochondrial dyes that, as a class, show some ability to stain living nerve terminals. Several of these agents provide excellent staining of neuromuscular junctions in a wide range of species. More detailed studies of the most effective of these dyes--4-(4-diethylaminostyryl)-N-methylpyridinium iodide (4-Di- 2-ASP)--indicate that it has no lasting effect on the structure or function of motor nerve terminals. As demonstrated in the accompanying paper (Lichtman et al., 1987; see also Lichtman et al., 1986), 4-Di-2- ASP can therefore be used to follow the configuration of identified motor terminals over arbitrarily long intervals.


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