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Journal of Neuroscience, Vol 7, 1752-1759, Copyright © 1987 by Society for Neuroscience
Recycling of plasmalemma in chick tectal growth cones
TP Cheng and TS Reese
Growth cones from freeze-substituted intact chick optic tectum were
analyzed in serial reconstructions of thin-section electron micrographs in
order to determine which internal organelles might contribute membrane for
plasmalemmal expansion. These growth cones contain numerous stacked and
single lumenless membrane-limited disks; the stacks are arrays of single
disks interconnected, and possibly organized, by intervening electron-dense
cross-links. The single and stacked disks together account for 80% of the
total intracellular membrane in the growth cones. Single disks frequently
lie close to and occasionally contact the filopodial plasmalemma; regularly
spaced electron-dense cross-links also occur at these juxtapositions
between single disks and the plasmalemma. Some of the juxtaposed disk
membranes contact the growth cone plasmalemma, and images of some of these
contacts appear to indicate fusion of the disk membrane with the
plasmalemma. When excised optic tecta are exposed to cationized ferritin
for various times, ferritin micelles appear sequentially in coated pits,
coated vesicles, smooth vesicles, vacuoles, and then in stacked and single
disks, including some of those contacting the plasmalemma. Because the
cytoplasmic disks filled only at the longest times after exposure to
ferritin, the membrane continuities between the disks and the plasmalemma
are thought to be indicative of exocytosis rather than endocytosis. We
propose, therefore, that components of the plasma membrane are recycled
through the stacks of lumenless disks in the chick tectal growth cones; the
disks therefore represent a pool of internal membrane waiting to be added
to the growth cone plasmalemma that could be used for filopodial extension
or neuritic extension.
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