WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience Fine Science Tools - Extraordinary Craftsmanship
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carlone, R.
Right arrow Articles by Rathbone, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carlone, R.
Right arrow Articles by Rathbone, M.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 7, 2163-2167, Copyright © 1987 by Society for Neuroscience

ARTICLE

Purification of a chick brain-derived growth factor by reversed-phase high-performance liquid chromatography

R Carlone, JK Kim and M Rathbone

A chick brain-derived growth factor (CBGF) has been purified to apparent homogeneity by a combination of ultrafiltration, DEAE ion- exchange, and reversed-phase high-performance liquid chromatography on a C18 column. CBGF has an apparent molecular mass of approximately 1500 Da. The amino acid composition of CBGF has been determined and reveals a peptide containing predominantly glycine, glutamic acid/glutamine, and aspartic acid/asparagine. This mitogen is highly active, with half- maximal stimulation of chick brain astrocytes at 5 ng/ml in an assay using incorporation of methyl-3H-thymidine into DNA. In addition to its effects on chick astrocytes, purified CBGF from the 18 d chick embryo is mitogenic for amphibian limb regeneration blastema cells in vitro. A possible role for this mitogen in the nerve-dependent regeneration of amphibian appendages is discussed.




-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-