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Journal of Neuroscience, Vol 7, 2232-2238, Copyright © 1987 by Society for Neuroscience
Serotonin produces long-term changes in the excitability of Aplysia sensory neurons in culture that depend on new protein synthesis
N Dale, ER Kandel and S Schacher
When isolated and grown in cell culture, the sensory and motor neurons of
the gill withdrawal reflex of Aplysia readily form synaptic connections.
Repeated exposures to 5-HT cause facilitation of the synaptic connections
between co-cultured sensory and motor neurons lasting at least 24 hr. As a
first step toward understanding the locus and the mechanisms underlying
this long-term synaptic facilitation, we have examined the membrane
excitability of the isolated presynaptic sensory neurons grown alone in
dissociated cell culture. Four repeated applications of 1 microM 5-HT
caused a significant increase in the excitability of sensory neurons,
lasting at least 24 hr. This resembles the short-term changes in
excitability seen in response to a single application of 5-HT. Unlike the
short-term effect, this long-lasting change was blocked by exposure of the
cells during the 5-HT treatment to 10 microM anisomycin, an inhibitor of
protein synthesis. Thus, like the synaptic facilitation, the long-term
change in excitability of the isolated presynaptic neurons differs from the
short-term in requiring the synthesis of new protein. This finding suggests
that the sensory neuron uses gene products to modulate membrane currents in
its long- term response to repeated external stimuli that are not required
in its short-term response to a single stimulus.
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