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Journal of Neuroscience, Vol 8, 4299-4306, Copyright © 1988 by Society for Neuroscience
Inward rectification of resting and opiate-activated potassium currents in rat locus coeruleus neurons
JT Williams, RA North and T Tokimasa
Vollum Institute, Oregon Health Sciences University, Portland 97201.
Intracellular recordings were made from rat locus coeruleus neurons in
vitro, and membrane currents were measured at potentials from -50 to - 130
mV. In the absence of any applied agonists, the slope conductance of the
cells increased 3-fold when the cell was hyperpolarized from -60 to -120
mV. This conductance increase was complete within 5 msec of the onset of a
hyperpolarizing command and was subsequently independent of time for
several seconds. The conductance increase was blocked by cesium chloride
(1-2 mM), rubidium chloride (1-2 mM), or barium chloride (1-100 microM).
The membrane potential range over which the conductance increased was
centered at the potassium equilibrium potential (EK; extracellular
potassium concentration, 2.5-10.5 mM): the current/voltage (I/V) relation
of the cell could be well described by supposing that there were 2
potassium conductances, one voltage independent (G1) and the other (inward
rectifier, Gir) activated according to the expression Gir = Gir,max/(1 +
exp[(V - EK)/k]), where k ranged from 15 mV in 2.5 mM potassium to 6 mV in
10.5 mM potassium. The additional membrane potassium conductance that
developed when agonists at mu-opioid and alpha 2-adrenoceptors were applied
also became larger with membrane hyperpolarization, and this voltage
dependence was also reduced or blocked by rubidium, cesium, and barium; in
the presence of these agonists the current also reached its final value
within 5 msec. However, the conductance increased by the agonists (Gag) was
not well expressed by simply increasing the values of G1 and Gir,max. It
was best described by a potassium conductance that increased according to
Gag,max/(1 + exp[(V - Vm)/k]), where Vm (the potential at which the
conductance was half-maximum) was close to the resting potential of the
cell.(ABSTRACT TRUNCATED AT 400 WORDS)
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