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Journal of Neuroscience, Vol 8, 4734-4744, Copyright © 1988 by Society for Neuroscience
Immunocytochemical localization of protein kinase C isozymes in rat brain
FL Huang, Y Yoshida, H Nakabayashi, WS Young 3d and KP Huang
Section on Metabolic Regulation, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Recently, we isolated 3 protein kinase C (PKC) isozymes from rat brain
(Huang et al., 1986a). Using isozyme-specific antibodies for immunoblot, we
have determined the relative levels of each isozyme in various regions of
the rat brain (Huang et al., 1987b). The present paper describes the
cellular distributions of PKC isozymes in rat brain as determined by light
microscopic immunocytochemistry. Staining with PKC antibodies revealed
strong immunoreactivities in neuronal somata and their dendrites and weak
to no reaction in axon and the astroglial structures. In the cerebellum,
the type I PKC antibodies stained the Purkinje cell bodies and dendrites;
the type II PKC antibodies stained the granule cells; and the type III PKC
antibody stained both Purkinje and granule cells. In the cerebral cortex,
all antibodies stained neurons resembling pyramidal cells and their apical
dendrites in layers II to VI, while layer I was nearly devoid of staining.
However, the various isozyme-specific antibodies revealed distinct laminar
distribution patterns of the positively stained neurons, and the type III
PKC-positive neurons exhibited a higher density than those of type I or II
PKC-positive ones, especially in layer II of cingulate (retrosplenial) and
piriform cortices. In the hippocampal formation, both pyramidal cells of
the hippocampus and granule cells of the dentate gyrus were stained by all
PKC antibodies. Subcellularly, type III PKC appeared mostly in the
cytoplasm of these neurons, whereas type I and II PKC seemed to associate
with the nucleus as well. In the olfactory bulb, both type II and III PKC
antibodies stained the periglomerular and granular cells, and the latter
also stained the mitral cells. The distinct cellular and subcellular
distribution of PKC isozymes suggests that each isozyme plays a unique role
in the various neural functions.
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