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Journal of Neuroscience, Vol 8, 3499-3506, Copyright © 1988 by Society for Neuroscience
Voltage- and ligand-activated inwardly rectifying currents in dorsal raphe neurons in vitro
JT Williams, WF Colmers and ZZ Pan
Vollum Institute, Oregon Health Sciences University, Portland 97201.
Intracellular recordings were made from neurons in rat dorsal raphe in the
slice preparation maintained at 37 degrees C. The single-electrode
voltage-clamp method was used to measure membrane currents at potentials
more negative than rest (-60 mV). Three types of inward rectification were
observed: 2 in the absence of any drugs and the third induced by 5-HT 1 and
GABA-B receptor agonists. In the absence of any drugs, an inward current
activated over 1-2 sec when the membrane potential was stepped to
potentials more negative than -70 mV. This current was blocked by cesium (2
mM) and resembles IQ or IH. A second inward current (IIR) occurred at
membrane potentials near the potassium equilibrium potential (EK). This
inward current activated within the settling time of the clamp and was
abolished by both barium (10-100 microM) and cesium (2 mM). 5-HT 1 agonists
activated a potassium conductance that hyperpolarized the cells at rest.
This potassium conductance was about 2 nS at -60 mV and increased linearly
with membrane hyperpolarization to about 4 nS at -120 mV. Baclofen
activated a potassium conductance identical in amplitude and voltage
dependence to that induced by 5-HT 1 agonists. Both the baclofen- and
5-HT-induced currents were nearly abolished in animals pretreated with
pertussis toxin. The results indicate that a common potassium conductance
is increased by 5-HT acting on 5-HT 1 receptors and baclofen acting on
GABA-B receptors. This potassium conductance rectifies inwardly and is
distinct from the Q-current. The ligand-activated potassium conductance
also differs from the other form of inward rectification (IIR) in its
voltage dependence and sensitivity to pertussis toxin.
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