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Journal of Neuroscience, Vol 9, 248-257, Copyright © 1989 by Society for Neuroscience
In situ hybridization analysis of myelin gene transcripts in developing mouse spinal cord
C Jordan, V Friedrich Jr and M Dubois-Dalcq
Laboratory of Molecular Genetics, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, Maryland 20892.
We analyzed the location and abundance of transcripts for the 4 CNS myelin
protein genes, myelin basic protein (MBP), proteolipid protein (PLP),
myelin-associated glycoprotein (MAG), and 2',3'-cyclic nucleotide
phosphohydrolase (CNP), in the mouse cervical spinal cord from the time of
rapid myelination until adulthood (8-45 d). In the white matter, maximal
levels of transcripts were found for each of the myelin genes at the peak
of myelination (8 d). Total MBP and PLP mRNAs stayed high until 20 d and
showed a minor decrease thereafter. In contrast, MAG and the MBP exon 2
containing transcripts (coding for the 21.5 and 17 kDa MBP isoforms)
decreased sharply between 8 and 20 d, suggesting that high levels of these
transcripts are needed primarily during the initiation of myelination. CNP
transcripts were less abundant, maintained high expression until 20 d, and
then decreased sharply. PLP, MAG, and CNP transcripts were clustered in the
oligodendrocyte cell body, while MBP mRNAs were scattered throughout the
cell body and processes. In contrast to the white matter, all these myelin
specific transcripts in the gray matter showed a marked increase from 8 to
20 d, as did the number of oligodendrocytes identified by CNP
immunostaining. MAG transcripts were found in white matter and in satellite
and other oligodendrocytes of the gray matter but not in neurons identified
by their expression of neurofilament transcripts. The results of our
quantitative in situ hybridization study are in good agreement with those
of previous molecular studies and provide new information on the cellular
and topographic distribution of myelin- specific mRNAs during myelination.
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