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Journal of Neuroscience, Vol 9, 1314-1324, Copyright © 1989 by Society for Neuroscience
Gating scheme for single GABA-activated Cl- channels determined from stability plots, dwell-time distributions, and adjacent-interval durations
DS Weiss and KL Magleby
Department of Physiology and Biophysics, University of Miami School of Medicine, Florida 33101.
To study the gating of a GABA-activated Cl- channel, currents from single
channels activated by 1.0 microM GABA were examined in patches of membrane
excised from cultured chick cerebral neurons. The distributions of open and
shut interval durations were each described by the sum of 3 exponential
components, suggesting that the channel normally enters at least 3 open and
3 shut states. Five different 6- state gating schemes were found that could
describe, all equally well, the observed distributions of open and shut
interval durations. Plots of the mean duration of open intervals adjacent
to shut intervals of specified durations revealed that, on the average,
openings of brief duration were adjacent to closings of long duration. This
observation indicated 2 or more independent transition pathways between the
open and shut states. Examination of the distributions of open intervals
adjacent to shut intervals of specified durations revealed that the time
constants of the exponential components describing these conditional
open-interval distributions were independent of the durations of the
adjacent shut intervals. In contrast, the areas changed in a manner
consistent with open states of briefer mean lifetimes typically making
transitions to shut states of longer mean lifetimes. Four of the 5 gating
schemes considered were rejected because they did not predict the
relationship between adjacent intervals or because they predicted that the
channel should switch between 2 gating modes with markedly different mean
open and shut times, which was not a characteristic of the experimental
data. The single remaining kinetic scheme could account for the observed
kinetic properties of the GABA channel.
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