 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 9, 2718-2736, Copyright © 1989 by Society for Neuroscience
Evidence for glycine as an inhibitory neurotransmitter of vestibular, reticular, and prepositus hypoglossi neurons that project to the cat abducens nucleus
RF Spencer, RJ Wenthold and R Baker
Department of Anatomy, Medical College of Virginia, Richmond 23298.
The localization and distribution of brain-stem afferent neurons to the cat
abducens nucleus has been examined by high-affinity uptake and retrograde
transport of 3H-glycine. Injections of 3H-glycine selectively labeled (by
autoradiography) only neurons located predominantly in the ipsilateral
medial vestibular and contralateral prepositus hypoglossi nuclei, and in
the contralateral dorsomedial reticular formation, the latter corresponding
to the location of inhibitory burst neurons. The specificity of uptake and
retrograde transport of 3H-glycine was indicated by the absence of labeling
of the dorsomedial medullary reticular neurons ipsilateral and in close
proximity to the injection site, where local uptake by diffusion could have
occurred. The selectivity of uptake and transport was demonstrated by the
absence of retrograde labeling following injections of 3H-GABA or
3H-leucine into the abducens nucleus. The immunohistochemical localization
of glycine and GABA revealed a differential distribution of the 2
inhibitory neurotransmitter candidates in the extraocular motor nuclei.
Glycine-immunoreactive staining of synaptic endings in the abducens nucleus
was dense with a widespread soma-dendritic distribution but was sparse in
the trochlear and oculomotor nuclei. By contrast, GABA-immunoreactive
staining within the oculomotor and trochlear nuclei was associated with
synaptic endings that were particularly prominent on the somata of
motoneurons. GABA- immunoreactive staining in the abducens nucleus,
however, was sparse. These differences between glycine- and
GABA-immunoreactive staining in the extraocular motor nuclei were
correlated with differences in the immunoreactivity of axons in the
descending (glycine) and ascending (GABA) limbs of the medial longitudinal
fasciculus. Glycine- immunoreactive neurons, furthermore, were observed in
the same locations as neurons that were labeled autoradiographically by
retrograde transport of 3H-glycine from the abducens nucleus.
Electrophysiological recordings from abducens motoneurons and internuclear
neurons revealed a marked reduction in the slow positivity of the
orthodromic extracellular potential elicited by ipsilateral vestibular
nerve stimulation following systemic administration of strychnine, an
antagonist of glycine. Intracellular recordings demonstrated that the
vestibular-evoked disynaptic inhibitory postsynaptic potentials in abducens
neurons were effectively blocked by strychnine but were unaffected by
picrotoxin, an antagonist of GABA.(ABSTRACT TRUNCATED AT 400 WORDS)
This article has been cited by other articles:
|
|
|
|
 
M. W. Bagnall, B. Zingg, A. Sakatos, S. H. Moghadam, H. U. Zeilhofer, and S. d. Lac
Glycinergic Projection Neurons of the Cerebellum
J. Neurosci.,
August 12, 2009;
29(32):
10104 - 10110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Iwamoto, H. Kaneko, K. Yoshida, and H. Shimazu
Role of Glycinergic Inhibition in Shaping Activity of Saccadic Burst Neurons
J Neurophysiol,
June 1, 2009;
101(6):
3063 - 3074.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Jones and M. Ariel
Morphology, Intrinsic Membrane Properties, and Rotation-Evoked Responses of Trochlear Motoneurons in the Turtle
J Neurophysiol,
March 1, 2008;
99(3):
1187 - 1200.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Ney, A. K. Hull, S. C. van Calcar, X. Liu, and M. R. Etzel
Dietary Glycomacropeptide Supports Growth and Reduces the Concentrations of Phenylalanine in Plasma and Brain in a Murine Model of Phenylketonuria
J. Nutr.,
February 1, 2008;
138(2):
316 - 322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Bagnall, R. J. Stevens, and S. du Lac
Transgenic Mouse Lines Subdivide Medial Vestibular Nucleus Neurons into Discrete, Neurochemically Distinct Populations
J. Neurosci.,
February 28, 2007;
27(9):
2318 - 2330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bergquist, A. Ruthven, M. Ludwig, and M. B. Dutia
Histaminergic and glycinergic modulation of GABA release in the vestibular nuclei of normal and labyrinthectomised rats
J. Physiol.,
December 15, 2006;
577(3):
857 - 868.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Takazawa, Y. Saito, K. Tsuzuki, and S. Ozawa
Membrane and Firing Properties of Glutamatergic and GABAergic Neurons in the Rat Medial Vestibular Nucleus
J Neurophysiol,
November 1, 2004;
92(5):
3106 - 3120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Russier, I. L Kopysova, N. Ankri, N. Ferrand, and D. Debanne
GABA and glycine co-release optimizes functional inhibition in rat brainstem motoneurons in vitro
J. Physiol.,
May 15, 2002;
541(1):
123 - 137.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Roy and K. E. Cullen
Vestibuloocular Reflex Signal Modulation During Voluntary and Passive Head Movements
J Neurophysiol,
May 1, 2002;
87(5):
2337 - 2357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Moreno-Lopez, M. Escudero, and C. Estrada
Nitric oxide facilitates GABAergic neurotransmission in the cat oculomotor system: a physiological mechanism in eye movement control
J. Physiol.,
April 1, 2002;
540(1):
295 - 306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Graf, R. Spencer, H. Baker, and R. Baker
Vestibuloocular Reflex of the Adult Flatfish. III. A Species-Specific Reciprocal Pattern of Excitation and Inhibition
J Neurophysiol,
September 1, 2001;
86(3):
1376 - 1388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. E. Angelaki, A. M. Green, and J. D. Dickman
Differential Sensorimotor Processing of Vestibulo-Ocular Signals during Rotation and Translation
J. Neurosci.,
June 1, 2001;
21(11):
3968 - 3985.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Aksay, R. Baker, H. S. Seung, and D. W. Tank
Anatomy and Discharge Properties of Pre-Motor Neurons in the Goldfish Medulla That Have Eye-Position Signals During Fixations
J Neurophysiol,
August 1, 2000;
84(2):
1035 - 1049.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. D. Kaufman, M. E. Shinder, and A. A. Perachio
Convergent Properties of Vestibular-Related Brain Stem Neurons in the Gerbil
J Neurophysiol,
April 1, 2000;
83(4):
1958 - 1971.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Straka, S. Biesdorf, and N. Dieringer
Canal-Specific Excitation and Inhibition of Frog Second-Order Vestibular Neurons
J Neurophysiol,
September 1, 1997;
78(3):
1363 - 1372.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Graf, R. Spencer, H. Baker, and R. Baker
Excitatory and Inhibitory Vestibular Pathways to the Extraocular Motor Nuclei in Goldfish
J Neurophysiol,
May 1, 1997;
77(5):
2765 - 2779.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Moreno-Lopez, M. Escudero, and C. Estrada
Nitric oxide facilitates GABAergic neurotransmission in the cat oculomotor system: a physiological mechanism in eye movement control
J. Physiol.,
April 1, 2002;
540(1):
295 - 306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
