Fig. 6. QKI-6, but not QKI-5, is absent from oligodendrocytes in qkv
optic nerve. Double immunolabeling of P14 mouse optic nerve with antisera against QKI-6 (green, a, b, d, e) or QKI-5 (green, c, f) and GFAP (red, a–c) or histones (red, d, e, g). a, In wild-type optic nerve, QKI-6 is located in GFAP−oligodendrocytes (arrowheads) as well as GFAP+ astrocytes (arrows). By striking contrast, in qkv
optic nerve (b) QKI-6 immunoreactivity is seen only in GFAP+ astrocytes (arrows); oligodendrocytes are devoid of QKI-6.c, However, QKI-5 immunoreactivity is present inqkv
optic nerve GFAP−oligodendrocytes (arrowheads), as well as in GFAP+ astrocytes (arrows). In wild-type optic nerve, double labeling with histones reveals that virtually all cells are QKI-6+ (d), but in theqkv
mutant, many histone+/QKI-6− cells (e,arrowheads) can be seen. As in wild-type, however, virtually all cells in the qkv
optic nerve, as labeled with histone antibodies (g), are QKI-5+ (f). a, d, wild-type, wt; b, c, e, f, g,qkv
, qk.