Fig. 1. Somatotopically appropriate 2DG labeling in normal hamster barrel field. Top, Low-magnification photomicrograph of a 2DG-labeled/GAD-stained section from hamster, including barrel cortex, cut tangential to the barrel representation in layer IV. The image was contrast-enhanced to show the pattern of 2DG label, which reveals a full body map of 2DG label (a “hamsterunculus”), including the whisker-related barrel field, in somatosensory cortex. Note the clear demarcation of individual barrels near the center of the section, marked A–E to denote barrel rows corresponding to rows of whiskers on the contralateral face. V, Visual cortex; T, trunk representation; F, forelimb;H, hindlimb; LL, lower lip. Scale bar, 500 μm.Bottom, A similar low-magnification photomicrograph of a Nissl-stained section from another hamster, also cut tangential to the barrels. Individual barrels are defined clearly with this stain, which is denser in barrel walls than in the septum between barrels. This pattern is similar to that shown with 2DG.