Fig. 5. BDNF in situ hybridization in L5 DRG, spinal cord, and sciatic nerve proximal to a ligation.A, Untreated DRG; B, sense strand hybridization control of ipsilateral DRG 1 d after sciatic crush;C, D, ipsilateral and contralateral DRG, respectively, 1 d after sciatic crush; E, F, ipsilateral and contralateral DRG, respectively, 1 d after cutting the sciatic nerve; G, H, ipsilateral and contralateral ventral horn, respectively, 1 d after cutting the sciatic nerve;I, sense strand hybridization control of nerve segment proximal to an 18–20 hr double ligation; J, proximal nerve segment removed immediately after placement of a double ligation;K, proximal nerve segment removed 18–20 hr after ligation of the sciatic nerve 1 d after sciatic nerve crush distal to the ligation site. Scale bar, 100 μm.