Fig. 2. Corresponding hippocampal sections 7 d after sham operation. Comparison of Nissl staining (A), immunocytochemistry (B–D), and nucleotidase histochemistry (E–H). A, Nissl staining revealed no neuronal damage in the hippocampus. B, OX42 immunostaining is significant only in fiber tracts, as for example in the corpus callosum. C, Vimentin is detectable in capillaries. D, GFAP immunoreaction is strongest in the stratum lacunosum moleculare. E–G, ATPase (E), UTPase (F), and ADPase (G) reaction product in the hippocampus is restricted mainly to capillaries. Staining is enhanced slightly in the stratum lacunosum. H, 5′-Nucleotidase activity is highest in CA1 and in the stratum lacunosum moleculare of CA1 and CA3. The pyramidal cell layers and the dentate granule cell layer are free of reaction product. CA1, CA3, Subfields of the hippocampus; cc, corpus callosum;gr, granular cell layer of the dentate gyrus;H, hilus of the dentate gyrus; lm, stratum lacunosum moleculare; pyr, stratum pyramidale;PC, plexus choroideus; M, meninx. Scale bar, 1 μm.