Fig. 1. Astrocyte stimulation increases mEPSC frequency. A, mEPSCs recorded at a holding potential of −60 mV before and after mechanical stimulation of an astrocyte.B, Time course of the mEPSC frequency calculated in 1 sec bins. Zero time corresponds to the time of astrocyte stimulation.C, Cumulative probability plot of the mEPSC frequency 30 sec before and after astrocyte stimulation (open andfilled symbols, respectively). D,E, Histograms of mEPSC amplitudes (bin width, 1 pA) recorded 30 sec before and after astrocyte stimulation, respectively.F, Cumulative probability plot of the mEPSC amplitudes recorded 30 sec before and after astrocyte stimulation (open and filled symbols, respectively).G, Average (n = 24) cumulative probability plot of the mEPSC amplitudes recorded 30 sec before and after astrocyte stimulation (open and filled symbols, respectively). Error bars showing SEM are smaller than symbol size. To obtain cumulative probability plots, we calculated the frequency and the amplitudes of mEPSCs in 1 sec and 1 pA bins, respectively.