Fig. 3. Intracellular distribution of rStau during the development of polarity in rat hippocampal neurons. mStau labeling is present in the cell body of stage 1 cells (a) and in the cell body and all neurites of stage 2, 3, and 4 (c, e, g, respectively). b, d,f, h, and j show all the processes of the cells identified by α-tubulin. At stage 5 (i), mStau becomes excluded from some of the thin, α-tubulin-positive processes (j,arrowheads). In this experiment, rat hippocampal neurons for 2, 4, and 8 d in culture have been used.