Fig. 1. Effects of BMP2 on the growth of cortical VZ cells plated at low density with FGF2 10 ng/ml. Photomicrographs of rat E13 cortical cells grown with FGF2 alone (A,D, G, J), FGF2 plus BMP2 10 ng/ml (B, E, H,K), or FGF2 plus BMP2 100 ng/ml (C, F, I,L). A–C, Phase-contrast images at 5 div.D–F, Bisbenzimide staining at 8 div.G–I, β tubulin III staining at 8 div.J–L, Nestin staining at 8 div. The same photographic field is shown for each condition in D,G, J; E, H,K; and F, I,L at 8 div. Arrows indicate examples of dying cells with apoptotic bodies (A–C), cells expressing β tubulin III-immunoreactivity (G–I), and nestin immunoreactivity (J–L). In each condition, an occasional cell coexpressed both β tubulin III and nestin (for example, thetop cell indicated with an arrow inI and L). Scale bar: A–C, 8 μm; D–L, 12 μm.