Fig. 2. LPS-induced IL-1β-IR in tissue associated with the abdominal vagus nerve. A, Cells in perivagal NALC expressing IL-1β-IR (dark cytoplasmic stainingindicated with arrows) 45 min after intraperitoneal LPS administration. B–D, IL-1β-IR dendritiform cells in connective tissue adjacent to the abdominal vagus nerve 60 min after LPS. B shows a high magnification of an IL-1β-IR dendritiform cell. E, F, Darkly stained IL-1β-positive cells in perivagal NALC (E, F, arrow), most of which are round in shape. Some dendritiform IL-1β-IR cells are present between nerve fibers at edge of vagus nerve (F,arrowheads). Scale bars: A, 25 μm;B, 10 μm; C, 50 μm; D, 25 μm; E, 50 μm; F, 50 μm.NALC, Nerve-associated lymphoid/myeloid cells;VN, vagus nerve.