Fig. 2. Distribution of GABAA receptor subunits α1, α2, α3, α5, and γ2 in sham-operated (A) and freeze-lesioned (B) rats. Color-coded images from immunohistochemically processed sections. For each subunit, the optical density of the immunoreactivity product was color-coded using a standard 256-level scale, ranging fromblack for background to violet,blue, green, yellow, andred for the most intense signals. Sham-operated rats (A) show the typical distribution pattern of subunits α1, α2, α3, α5, and γ2 with symmetric intensities on both hemispheres. Animals with freeze-lesion-induced cortical malformations (B, microgyrus marked with anarrow) display widespread reduction in immunoreactivity for all subunits, most prominently for subunits α1 and γ2, involving the area of the dysplastic cortex, but also surrounding neocortical areas and the ipsilateral hippocampal formation (see Fig.6).