Fig. 3. Localization of ADAM8 in glia cells in the spinal cord of 40-d-old WT (A–C, G–I) and WR (D–F, J–L) mice, as demonstrated by immunofluorescence. A–C, WT, anti-ADAM8 (green, A), anti-CD45 (red, B), or both (yellow, C); insetsin A–C, staining of cells in the white matter with anti-ADAM8 (in A), oligodendrocyte-specific antigen CNPase (in B) and merged (in C).D–F, WR, ADAM8 (green,D), CD45 (red, E), or both (yellow, F). Note that all microglia cells express ADAM8. G–I, Spinal cord of WT mouse, stained with anti-ADAM8 (G), GFAP (H), or with both (I). J–L, WR, stained for ADAM8 (J), GFAP (K), or both (L). Note that, in J–L, astrocytes appear more compacted than usual because of very short exposure times. GFAP staining in astrocytic processes is not visible. Scale bar (in A): A–L, 50 μm.