Fig. 5. Reduction of Cl− outward transport by inhibitors of PTK. A, In a type 1 neuron, lavendustin A (10 μm) had an effect on sIPSCs comparable with furosemide. B, The ratio of outward to inward sIPSCs normalized to control was significantly reduced during application of lavendustin A (10 μm; p ≤ 0.001) or genistein (50 μm for 30 sec; p≤ 0.001) but not by daidzein (50 μm for 30 sec), the inactive analog of genistein. C, In type 2 neurons, inward currents and outward currents normalized to control at the respective VH were reduced, but the ratio did not change. D1, Currents induced by focal applications of GABA (1 mm) to a dendrite decreased or reversed in direction, respectively, when genistein (50 μm) or furosemide (100 μm) were applied to a type 1 neuron (VH of −75 mV). AtEGABA(VH of −85 mV), there was an increase in inward currents in genistein and furosemide. D2, The current–voltage relationship for the neuron shown in D1revealed a positive shift in EGABA(ΔVrev) in genistein (▪) and furosemide (●). Control, ■; washout, ▿. E, Correlation of ΔVrev induced by genistein (50 μm) and furosemide (100 μm) in 12 neurons was highly significant (r = 0.98). con, Control; daidz, daidzein; furo, furosemide; genist, genistein;lav A, lavendustin A; wash, washout.