Fig. 1. DSP4 induces NE depletion and LC cell loss. Rats received two injections of DSP4 1 week apart (A), followed 4 weeks later by intracortical injection of Aβ or saline (B). Serial sections from DSP4-treated rats (C, E, G) and control rats (D, F, H) were prepared 1 d later for immunocytochemistry and stained for NE (C, D) or TH (E–H). C, D, Sections from the frontal cortex. E, F, Sections from the area of the LC.G, H, Sections from the area of the substantia nigra. Staining was performed on sections obtained from three animals in each group; representative images are shown. Scale bars: C,D, 25 μm; E–H, 50 μm.