Fig. 3. Eight weeks after injection α-synuclein-positive granular inclusions (A, B, arrowheads) and discrete intracytoplasmic inclusions where α-synuclein immunoreactivity occurred as a halo around a pale core (C, D, arrowheads) appear in large numbers in the cells of SNc, associated with swollen dystrophic α-synuclein-positive neurites (B,E, F) and atrophic perikarya (F). Arrows in B,E, F, and G indicate intact neurites of normal size; G, H, none of these changes occurred in GFP overexpressing SNc neurons;I–K, the α-synuclein-immunoreactive terminals in the striatum had a normal morphology at 3 weeks (I) but were reduced in number and displayed large numbers of swollen, dystrophic profiles at 8 weeks (J, P, arrowheads). At 27 weeks, fewer dystrophic fibers were seen, but the density of α-synuclein-positive terminals remained reduced (K); L, none of these axonal changes were observed in the GFP transduced controls, although GFP expression was maintained at high levels still by 27 weeks;M–Q, examples of swollen, dystrophic axon terminals in the striatum, revealed by TH (M–O) and α-synuclein antibodies (P, Q); R,S, expression of α-synuclein did not induce any pathological changes in nondopaminergic projections to the superior colliculus (R) and thalamus (S) (8 week survival). Scale bars: A,10 μm (applies to A–H, O–Q); I, 50 μm (applies to I–L, R, S); M, 20 μm (applies toM, N).