Figure 1. Immunoblot analysis of αCaMKII expression in the somatosensory cortex (Sm Cx) and hippocampus (Hi) in αCaMKII T286A mutants (T286A; n = 3) and wild-type littermates (wt; n = 3). The samples from three mutants (t286A-1, t286A-2, and t286A-3) and three wild-type littermates (wt-1, wt-2, and wt-3) are shown. A, The immunoblots were used first for detecting αCaMKII expression, and, after stripping of the blots, β-actin expression was determined as a standard for normalization. B, Expression in the somatosensory cortex was quantified and plotted in reference to expression in wild-type littermates (white bars). There was only a trend to suggest that αCaMKII expression was reduced in the mutants (F(1,4) = 6.29; p = 0.066), whereas αCaMKII expression normalized to β-actin expression was significantly reduced by 25% (F(1,4) = 13.7; p < 0.05) C, Expression in the hippocampus was quantified and plotted in reference to expression in wild-type littermates (white bars). The αCaMKII expression in the mutants was significantly reduced by 17% (F(1,4) = 9.09; p < 0.05), but this reduction could not be confirmed when the αCaMKII expression was normalized to β-actin expression (F(1,4) = 0.002; p = 0.96). Error bars depict SEMs.