Fig. 1. Time course of MAP kinase activation.A, Time course of MAPK activation by five spaced pulses of 5-HT. Pleural-pedal ganglia were treated with five pulses of 5-HT (5×5HT), SN clusters were excised either immediately (0 h) or 1 (1 h), 3 (3 h), or 20 (20 h) hr after the last pulse of 5-HT, and MAPK activation was examined using phospho-MAPK and phospho-independent MAPK antibodies. A1, Sample blots with phospho-MAPK and total MAPK antibodies.A2
, Summary of time course of MAPK activation by five spaced pulses of 5-HT. Data are presented as mean ± SEM (percentage of control; n = 12 per group, each time point). The 5-HT-treated samples are normalized to their paired ASW controls at each time point. B, Time course of MAPK activation by five spaced tail shocks. Animals were given five spaced shocks (5×TS) to one side of the tail, SN clusters were excised at the indicated times, and MAPK activation was examined as described above. B1
, Sample blots with phospho-MAPK and total MAPK antibodies.B2
, Summary of time course of MAPK activation by five spaced tail shocks. Data are presented as mean ± SEM [percentage of control; n = 8 (1 hr), 7 (3 hr), and 6 (22 hr)]. The shocked side samples are normalized to their paired nonshocked control side samples at each time point.